Stothard J R, Llewellyn-Hughes J, Griffin C E, Hubbard S J, Kristensen T K, Rollinson D
Wolfson Wellcome Biomedical Laboratories, Department of Zoology, The Natural History Museum, London.
Mem Inst Oswaldo Cruz. 2002;97 Suppl 1:31-6. doi: 10.1590/s0074-02762002000900008.
Identification of populations of Bulinus nasutus and B. globosus from East Africa is unreliable using characters of the shell. In this paper, a molecular method of identification is presented for each species based on DNA sequence variation within the mitochondrial cytochrome oxidase subunit I (COI) as detected by a novel multiplexed SNaPshotTM assay. In total, snails from 7 localities from coastal Kenya were typed using this assay and variation within shell morphology was compared to reference material from Zanzibar. Four locations were found to contain B. nasutus and 2 locations were found to contain B. globosus. A mixed population containing both B. nasutus and B. globosus was found at Kinango. Morphometric variation between samples was considerable and UPGMA cluster analysis failed to differentiate species. The multiplex SNaPshotTM assay is an important development for more precise methods of identification of B. africanus group snails. The assay could be further broadened for identification of other snail intermediate host species.
利用贝壳特征来鉴定东非的鼻泡螺和球泡螺种群并不可靠。本文基于线粒体细胞色素氧化酶亚基I（COI）内的DNA序列变异，通过一种新型多重SNaPshotTM检测法，为每个物种提出了一种分子鉴定方法。总共使用该检测法对来自肯尼亚沿海7个地点的蜗牛进行了分型，并将贝壳形态的变异与来自桑给巴尔的参考材料进行了比较。发现有4个地点含有鼻泡螺，2个地点含有球泡螺。在基南戈发现了一个同时包含鼻泡螺和球泡螺的混合种群。样本之间的形态计量变异相当大，UPGMA聚类分析未能区分物种。多重SNaPshotTM检测法是更精确鉴定非洲泡螺组蜗牛方法的一项重要进展。该检测法可进一步扩展用于鉴定其他蜗牛中间宿主物种。
