Alharbi Mohammad H, Iravoga Cynthia, Kayuni Sekeleghe A, Cunningham Lucas, LaCourse E James, Makaula Peter, Stothard J Russell
Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Ministry of Health, Buraydah 52367, Saudi Arabia.
Trop Med Infect Dis. 2022 Aug 19;7(8):195. doi: 10.3390/tropicalmed7080195.
The freshwater snail genus plays a vital role in transmitting parasites of the group. A hybrid schistosome between and has been recently detected using DNA-based identification methods in school children along the Lake Malawi shoreline in Mangochi District. This finding raised the need for contemporary revaluation of local interactions between schistosomes and snails, with a particular focus on snail species within the group. In 2017 and 2018, malacological surveys sampled several freshwater sites in Mangochi District. Collected snails ( = 250) were characterised using cytochrome oxidase subunit 1 gene (1), with DNA barcoding of the 'Folmer' region and a rapid PCR-RFLP typing assay with double digestion with III and I restriction enzymes. DNA 1 sequence analysis, with phylogenetic tree construction, suggested the presence of at least three group taxa in Lake Malawi, alongside first reports of and which can be differentiated by PCR-RFLP methods. In addition, a total of 30 of the 106 group snails (28.30%) were positive to the -specific screen using real-time PCR methods. This study provides new insight into the recent changes in the epidemiology of urogenital schistosomiasis as likely driven by a new diversity of group snails within the Lake.
淡水螺属在传播该类寄生虫方面起着至关重要的作用。最近，在曼戈奇区马拉维湖沿岸的学童中，利用基于DNA的鉴定方法检测到了一种 与 之间的杂交血吸虫。这一发现引发了对血吸虫与蜗牛之间局部相互作用进行当代重新评估的需求，尤其关注该类群中的蜗牛种类。2017年和2018年，进行了软体动物学调查，对曼戈奇区的几个淡水地点进行了采样。使用细胞色素氧化酶亚基1基因（1）对采集的蜗牛（ = 250）进行了特征分析，采用了“福尔默”区域的DNA条形码技术以及用III和I限制性内切酶进行双酶切的快速PCR-RFLP分型检测。DNA 1序列分析以及系统发育树构建表明，马拉维湖至少存在三种该类群的分类单元，同时首次报告了 和 ，它们可以通过PCR-RFLP方法进行区分。此外，在106只该类群蜗牛中，共有30只（28.30%）通过实时PCR方法对特定筛查呈阳性。这项研究为泌尿生殖系统血吸虫病流行病学的近期变化提供了新的见解，这种变化可能是由该湖内该类群蜗牛的新多样性所驱动的。
