Ginsel L A, Onderwater J J, Daems W T
Histochemistry. 1979 Jul 11;61(3):343-6. doi: 10.1007/BF00508456.
The electron-microscopical autoradiographical resolution of a gold latensification-elon ascorbic acid (GEA) developer for Ilford L4 emulsion was determined experimentally, using radioactive line sources of tritiated albumin (Heijnen and Geuze, 1977). For sections with a thickness of 62 nm (SD +/- 11), which were covered with a carbon layer about 5 nm thick and a slightly overlapping monolayer of L4 silver bromide crystals, the measured half-distance (HD) of resolution was 115 nm. This improvement in resolution, the high efficiency of the GEA developer for L4 emulsion (Wisse and Tates, 1968), and the excellent visibility of the cellular structures under the small silver grains, mean that the L4-GEA combination deserves preverence as a method for quantitative electron-microscopical autoradiography.
使用氚化白蛋白的放射性线源（海伊嫩和格兹，1977年），通过实验确定了用于伊尔福L4乳剂的金增感-伊伦抗坏血酸（GEA）显影剂的电子显微镜放射自显影分辨率。对于厚度为62纳米（标准差±11）、覆盖有约5纳米厚的碳层和稍有重叠的单层L4溴化银晶体的切片，测得的分辨率半距离（HD）为115纳米。这种分辨率的提高、GEA显影剂对L4乳剂的高效性（维瑟和泰茨，1968年）以及在小银粒下细胞结构的极佳可见性，意味着L4-GEA组合作为一种定量电子显微镜放射自显影方法值得优先选用。
