Barjas-Castro M L, Brandão M M, Fontes Adriana, Costa F F, Cesar C L, Saad S T O
Hematology and Transfusion Center, State University of Campinas, São Paulo, Brazil.
Transfusion. 2002 Sep;42(9):1196-9. doi: 10.1046/j.1537-2995.2002.00201.x.
Gamma irradiation of RBCs results in the production of reactive oxygen capable of initiating the process of membrane lipid peroxidation and accelerates the leakage of potassium ions from RBCs, resulting in an increase of internal viscosity.
The elastic properties of irradiated and stored RBC units were studied using laser optical tweezers. The laser trapped the cell and the membrane elasticity was analyzed, measuring the cell deformation in six different drag velocities. Five RBC units were split into two portions. One portion received a gamma irradiation dose of 25 Gy, and the second one was used as a control and was not irradiated. All units were stored (4 degrees C), and the elasticity was examined on Days 1, 14, 21, and 28.
Elastic properties (mu) from irradiated RBCs stored for 21 and 28 days were significantly affected compared with control cells (21 days: control, 0.3 +/- 0.03 x 10(-3); irradiated, 3.5 +/- 1.3 x 10(-3) dyn/cm; p < 0.001; and 28 days: control, 0.5 +/- 0.09 x 10(-3); irradiated, 14 +/- 3.2 x 10(-3) dyn/cm; p < 0.001).
The sensitivity of the laser optical tweezers method showed that there is no significant change in elasticity over time for up to 14 days of storage, regardless of whether the unit was irradiated or not. However, beyond 21 days of storage, irradiated units demonstrate decreased elasticity.
红细胞的γ射线辐照会产生能够引发膜脂质过氧化过程的活性氧，并加速钾离子从红细胞中泄漏，导致内部黏度增加。
使用激光光镊研究辐照和储存的红细胞单位的弹性特性。激光捕获细胞并分析膜弹性，测量细胞在六种不同拖曳速度下的变形。将五个红细胞单位分成两部分。一部分接受25 Gy的γ射线辐照剂量，另一部分用作对照，未进行辐照。所有单位均在4℃下储存，并在第1、14、21和28天检查弹性。
与对照细胞相比，储存21天和28天的辐照红细胞的弹性特性（μ）受到显著影响（21天：对照，0.3±0.03×10⁻³；辐照，3.5±1.3×10⁻³达因/厘米；p<0.001；28天：对照，0.5±0.09×10⁻³；辐照，14±3.2×10⁻³达因/厘米；p<0.001）。
激光光镊方法的敏感性表明，无论单位是否辐照，在储存长达14天的时间内，弹性均无显著变化。然而，储存超过21天后，辐照单位的弹性会降低。
