辐照红细胞细胞外钾离子浓度的动力学

Kinetics of extracellular potassium concentration in irradiated red blood cells.

作者信息

Arseniev L, Schumann G, Andres J

机构信息

Blutbank-Immunohämatologie-Transfusionsmedizin, Medizinishche Hochschule Hannover, FRG.

出版信息

Infusionsther Transfusionsmed. 1994 Oct;21(5):322-4. doi: 10.1159/000223002.

DOI:10.1159/000223002

PMID:7803994

Abstract

BACKGROUND

Irradiation of cellular blood components prevents onset of transfusion-associated graft-versus-host disease. The present study was designed to monitor the post-irradiation changes of the extracellular potassium concentration in red blood cells (RBC) stored in SAG-M (saline adenine glucose-mannitol), in order to estimate the right time for their prophylactic irradiation and the right span of post-irradiation storage.

MATERIALS AND METHODS

Ten units each of fresh, 10 days and more than 20 days stored SAG-M RBCs were divided into 2 equal portions each. One portion was gamma-irradiated with 30 Gy (137Cs-source). Another 10 fresh RBC units were separated, and portions were irradiated with 15, 30, 60 or 120 Gy. The determination of potassium was performed simultaneously in corresponding irradiated and non-irradiated portions; immediately after irradiation, after 1 and 5 days, and every 10 days of additional storage (up to 30 days). All measurements were performed in the cell-free supernatant, using flame emission photometry.

RESULTS

The extracellular potassium concentration increased permanently with the duration of storage both in non-irradiated and irradiated specimens. At the end of the period of storage this increase in the irradiated portions was approximately 2 times the potassium concentration of the non-irradiated portions (60 mmol/l vs. 38 mmol/l, p < 0.001). Thereby no significant differences of the potassium efflux rates were estimated at the end of the period of storage between fresh RBCs irradiated and stored RBCs irradiated. The mean 5 days post-irradiation potassium release from fresh RBC units was similar to that in 25-30 days stored non-irradiated portions (34.3 mmol/l vs. 38 mmol/l). The extracellular potassium increase was irradiation dose-dependent (r = 0.89, p < 0.001).

CONCLUSIONS

SAG-M RBCs can be irradiated with 30 Gy immediately post-harvest and subsequently stored for a couple of days prior to transfusion without producing critically high extracellular potassium concentrations.

摘要

背景

对细胞血液成分进行辐照可预防输血相关移植物抗宿主病的发生。本研究旨在监测储存在SAG-M（生理盐水腺嘌呤葡萄糖-甘露醇）中的红细胞（RBC）经辐照后细胞外钾浓度的变化，以确定其预防性辐照的合适时间以及辐照后储存的合适时长。

材料与方法

将10个单位的新鲜、储存10天及储存超过20天的SAG-M红细胞各分成2等份。其中一份用30 Gy（137Cs源）进行γ辐照。另外分离出10个新鲜红细胞单位，并将其分成若干份，分别用15、30、60或120 Gy进行辐照。在相应的辐照和未辐照部分同时进行钾含量测定；在辐照后立即、1天和5天后以及额外储存的每10天（直至30天）进行测定。所有测量均在无细胞上清液中使用火焰发射光度法进行。

结果

在未辐照和辐照样本中，细胞外钾浓度均随储存时间的延长而持续升高。在储存期结束时，辐照部分的这种升高约为未辐照部分钾浓度的2倍（60 mmol/L对38 mmol/L，p<0.001）。因此，在储存期结束时，新鲜红细胞辐照组和储存红细胞辐照组之间的钾外流速率没有显著差异。新鲜红细胞单位辐照后5天的平均钾释放量与储存25 - 30天的未辐照部分相似（34.3 mmol/L对38 mmol/L）。细胞外钾的增加呈辐照剂量依赖性（r = 0.89，p<0.001）。