Lee SangMyung, Min ByungSun, Kho YungHee
Korea Research Institute of Bioscience and Biotechnology, Daejon.
Arch Pharm Res. 2002 Oct;25(5):652-4. doi: 10.1007/BF02976939.
Brine shrimp assay-guided fractionation and isolation of the EtOAc soluble fraction of Phryma leptostachya L. (Phrymacaceae) gave two active compounds, phrymarolin II (1) and ursolic acid (2), which were identified by physicochemical and spectroscopic methods. Compound 1 exhibited potent lethality with LD50 value of 0.0013 microg/ml, whereas 2 showed moderate lethality with LD50 value of 27.0 microg/ml against brine shrimp. The cytotoxic activities of 1 and 2 were also evaluated against one murine and five human cancer cell lines employing the sulforhodamin B (SRB) method. Compound 2 exhibited cytotoxic activity against L1210 and SK-MEL-2 cells with ED50 values of 3.70 and 9.27 mg/ml, respectively, whereas 1 was devoid of any cytotoxic activity against all cancer cells tested.
卤虫生物测定法指导下对透骨草（透骨草科）乙酸乙酯可溶部分进行的分离和纯化得到了两种活性化合物，透骨草灵II（1）和熊果酸（2），通过物理化学和光谱方法对其进行了鉴定。化合物1表现出很强的致死性，对卤虫的半数致死量（LD50）值为0.0013微克/毫升，而化合物2表现出中等致死性，对卤虫的LD50值为27.0微克/毫升。还采用磺酰罗丹明B（SRB）法评估了化合物1和2对一种小鼠癌细胞系和五种人类癌细胞系的细胞毒性活性。化合物2对L1210和SK-MEL-2细胞表现出细胞毒性活性，其半数有效剂量（ED50）值分别为3.70和9.27毫克/毫升，而化合物1对所有测试的癌细胞均无任何细胞毒性活性。
