Deng Yan, Yang Liu, An Sheng-Li
Department of Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):1017-9.
To study the effects of the decoction of Tribulus terrestris L on tyrosinase activity and melanogenesis.
MTT was employed to study the proliferation of human melanocytes cultured in vitro, and tyrosinase activity was estimated by measuring the rate of oxidation of DL-dopa, after the cells were treated with different concentrations of decoction of Tribulus terrestris L.
The treatment with the decoction increased the amount of melanin at higher concentrations but act to the reverse effect at lower concentrations, with the best concentrations for promoting and inhibiting the cell growth being 1.5 mg/ml (P<0.05) and 0.5 mg/ml (P<0.05) respectively. At high concentrations the decoction enhanced the tyrosinase activity that was inhibited at low concentrations. The best concentrations for enhancing and inhibiting tyrosinase activity were 100 mg/ml (P<0.05) and 10 mg/ml (P<0.05) respectively.
Tribulus terrestris L decoction exercises a two-way regulation on the activity of tyrosinase and the proliferation of melanocytes.
研究刺蒺藜水煎剂对酪氨酸酶活性及黑素生成的影响。
采用MTT法研究体外培养的人黑素细胞的增殖情况，在用不同浓度的刺蒺藜水煎剂处理细胞后，通过测定DL-多巴的氧化速率来评估酪氨酸酶活性。
水煎剂处理在较高浓度时增加了黑色素的含量，但在较低浓度时则起相反作用，促进和抑制细胞生长的最佳浓度分别为1.5mg/ml（P<0.05）和0.5mg/ml（P<0.05）。在高浓度时，水煎剂增强了酪氨酸酶活性，而在低浓度时则受到抑制。增强和抑制酪氨酸酶活性的最佳浓度分别为100mg/ml（P<0.05）和10mg/ml（P<0.05）。
刺蒺藜水煎剂对酪氨酸酶活性和黑素细胞增殖具有双向调节作用。
