当归对体外黑素细胞增殖、黑色素合成及酪氨酸酶活性的影响。
Effect of Angelica sinensis (Oliv.) on melanocytic proliferation, melanin synthesis and tyrosinase activity in vitro.
作者信息
Deng Yan, Yang Liu
机构信息
Department of Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2003 Mar;23(3):239-41.
OBJECTIVE
To investigate the effects of Angelica sinensis (Oliv.) on melanocytes and tyrosinase activity.
METHODS
MTT method and cleavage by NaOH were employed to measure the proliferation and melanin synthesis of melanocytes, respectively. Tyrasinase activity assessment was performed by measuring the rate of oxidation of DL-dopa.
RESULTS
Angelica sinensis (Oliv.) was found to promote melanocytic proliferation (P < 0.05), resulting in a statistically significant increase in the cell counts (P < 0.05). It also enhanced melanin synthesis (P < 0.05) and tryosinase activity (P < 0.05) of the melanocytes, and the most potent effects were achieved at the concentration of 1 g/L (P < 0.05).
CONCLUSION
Angelica sinensis (Oliv.) promotes melanocytic proliferation, melanin synthesis and tyrosinase activity, which may be the mechanism for validating its clinical use in the treatment of skin pigmentation.
目的
研究当归对黑素细胞及酪氨酸酶活性的影响。
方法
分别采用MTT法和氢氧化钠裂解测定法检测黑素细胞的增殖及黑色素合成情况。通过测定DL-多巴的氧化速率评估酪氨酸酶活性。
结果
发现当归可促进黑素细胞增殖（P<0.05），细胞计数显著增加（P<0.05）。它还增强了黑素细胞的黑色素合成（P<0.05）及酪氨酸酶活性（P<0.05），在浓度为1g/L时效果最为显著（P<0.05）。
结论
当归可促进黑素细胞增殖、黑色素合成及酪氨酸酶活性，这可能是其在临床用于治疗皮肤色素沉着有效的作用机制。
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