Kosari Farhad, Asmann Yan W, Cheville John C, Vasmatzis George
Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
Cancer Epidemiol Biomarkers Prev. 2002 Nov;11(11):1419-26.
Electronic profiling of publicly available expressed sequence tag databases identified a gene, cysteine-rich secretoryprotein-3 (CRISP-3), that is up-regulated in prostate cancer, and of which the expression is relatively prostate-specific. The objective of this study was to examine the potential of CRISP-3 as a biomarker for prostate cancer. In transient transfection studies, CRISP-3 was found to be a secretory protein. Using a multiple tissue dot blot experiment, CRISP-3 transcript was identified in a limited number of human tissues including the prostate. In situ hybridization experiments indicated that CRISP-3 mRNA is epithelial-specific and is up-regulated in prostate adenocarcinoma compared with benign prostate tissue. CRISP-3 mRNA overexpression in cancer was confirmed using quantitative real-time reverse-transcription-PCR using benign prostatic epithelia and adenocarcinoma (in 5 of 5 cases) isolated by laser capture microdissection, as well as bulk tissues (in 20 of 23 cases) from surgically resected human prostates. These findings suggest that CRISP-3 is a potential biomarker for prostate cancer.
通过对公开可用的表达序列标签数据库进行电子分析，鉴定出一个基因——富含半胱氨酸分泌蛋白3（CRISP-3），该基因在前列腺癌中上调，且其表达相对具有前列腺特异性。本研究的目的是检验CRISP-3作为前列腺癌生物标志物的潜力。在瞬时转染研究中，发现CRISP-3是一种分泌蛋白。通过多组织斑点印迹实验，在包括前列腺在内的有限数量的人体组织中鉴定出了CRISP-3转录本。原位杂交实验表明，与良性前列腺组织相比，CRISP-3 mRNA具有上皮特异性，且在前列腺腺癌中上调。使用激光捕获显微切割分离的良性前列腺上皮和腺癌（5例中的5例）以及手术切除的人体前列腺的大块组织（23例中的20例），通过定量实时逆转录PCR证实了CRISP-3 mRNA在癌症中的过表达。这些发现表明CRISP-3是前列腺癌的潜在生物标志物。
