García Andrés J, Keselowsky Benjamin G
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta 30332, USA.
Crit Rev Eukaryot Gene Expr. 2002;12(2):151-62. doi: 10.1615/critreveukaryotgeneexpr.v12.i2.50.
Cell adhesion to extracellular matrix ligands through integrin receptors plays a central role in bone formation and maintenance by anchoring cells and triggering signals that direct osteoblast proliferation and differentiation. Moreover, osteoblast adhesion to adsorbed, synthesized, or engineered extracellular ligands on synthetic surfaces is critical to numerous biomedical and biotechnological applications. Considerable research efforts have concentrated on the development of surfaces that promote osteoblast differentiation and bone formation. Emerging surface engineering approaches have focused on creating biomimetic substrates that target integrins to activate signaling pathways directing the osteoblast differentiation program. These initiatives generally rely on controlling the adsorption of extracellular matrix ligands or engineering synthetic supports presenting bioadhesive motifs from extracellular matrix proteins. These biomolecular approaches provide promising strategies for the engineering of robust biofunctional matrices that control cell adhesion and signaling and promote osteoblast proliferation, differentiation, and matrix mineralization.
细胞通过整合素受体与细胞外基质配体的黏附在骨形成和维持过程中起着核心作用，它通过锚定细胞并触发指导成骨细胞增殖和分化的信号来实现。此外，成骨细胞与合成表面上吸附的、合成的或工程化的细胞外配体的黏附对于众多生物医学和生物技术应用至关重要。大量研究工作集中在开发促进成骨细胞分化和骨形成的表面。新兴的表面工程方法专注于创建仿生基质，这些基质靶向整合素以激活指导成骨细胞分化程序的信号通路。这些举措通常依赖于控制细胞外基质配体的吸附或工程化呈现细胞外基质蛋白生物黏附基序的合成载体。这些生物分子方法为构建强大的生物功能基质提供了有前景的策略，该基质可控制细胞黏附与信号传导，并促进成骨细胞增殖、分化和基质矿化。
