Sun Shuo, Jiao Zixue, Wang Yu, Wu Zhenxu, Wang Haowei, Ji Qingming, Liu Yi, Wang Zongliang, Zhang Peibiao
Department of Spine Surgery, The First Hospital of Jilin University, 1 Xinmin Street, Changchun 130021, China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Regen Biomater. 2021 Mar 19;8(2):rbab013. doi: 10.1093/rb/rbab013. eCollection 2021 Mar.
Porous microcarriers have aroused increasing attention recently by facilitating oxygen and nutrient transfer, supporting cell attachment and growth with sufficient cell seeding density. In this study, porous polyetheretherketone (PEEK) microcarriers coated with mineralized extracellular matrix (mECM), known for their chemical, mechanical and biological superiority, were developed for orthopedic applications. Porous PEEK microcarriers were derived from smooth microcarriers using a simple wet-chemistry strategy involving the reduction of carbonyl groups. This treatment simultaneously modified surface topology and chemical composition. Furthermore, the microstructure, protein absorption, cytotoxicity and bioactivity of the obtained porous microcarriers were investigated. The deposition of mECM through repeated recellularization and decellularization on the surface of porous MCs further promoted cell proliferation and osteogenic activity. Additionally, the mECM coated porous microcarriers exhibited excellent bone regeneration in a rat calvarial defect repair model , suggesting huge potential applications in bone tissue engineering.
多孔微载体通过促进氧气和营养物质的传递，以足够的细胞接种密度支持细胞附着和生长，近年来引起了越来越多的关注。在本研究中，开发了涂覆有矿化细胞外基质（mECM）的多孔聚醚醚酮（PEEK）微载体，其以化学、机械和生物学优势而闻名，用于骨科应用。多孔PEEK微载体是由光滑微载体通过一种简单的湿化学策略衍生而来，该策略涉及羰基的还原。这种处理同时改变了表面拓扑结构和化学成分。此外，还研究了所得多孔微载体的微观结构、蛋白质吸收、细胞毒性和生物活性。通过在多孔微载体表面反复进行再细胞化和去细胞化来沉积mECM，进一步促进了细胞增殖和成骨活性。此外，mECM涂层的多孔微载体在大鼠颅骨缺损修复模型中表现出优异的骨再生能力，表明在骨组织工程中具有巨大的潜在应用价值。
