Ziegler Annemarie, Zangemeister-Wittke Uwe, Stahel Rolf A
Departement für Innere Medizin, Onkologie Abteilung, Universitätsspital Zürich, Haeldeliweg 4, Switzerland.
Cancer Treat Rev. 2002 Oct;28(5):255-71. doi: 10.1016/s0305-7372(02)00077-4.
The recent discovery that cell-free DNA can be shed into the bloodstream as a result of tumour cell death has generated great interest. Numerous studies have demonstrated tumour-specific alterations in DNA recovered from plasma or serum of patients with various malignancies, a finding that has potential for molecular diagnosis and prognosis. The implication is that tumour-derived nucleic acids of human or viral origin can be retrieved from blood by a minimally invasive procedure, and used as a surrogate tumour marker to monitor the course of the disease or aid in early diagnosis. The present review will describe the main areas of ongoing investigation, with particular emphasis on technical issues and available data of clinical relevance.
最近发现，肿瘤细胞死亡可导致游离DNA释放入血液，这引起了人们极大的兴趣。大量研究已证实，从患有各种恶性肿瘤的患者的血浆或血清中回收的DNA存在肿瘤特异性改变，这一发现具有分子诊断和预后的潜力。这意味着可以通过微创程序从血液中获取人源或病毒源的肿瘤衍生核酸，并将其用作替代肿瘤标志物来监测疾病进程或辅助早期诊断。本综述将描述正在进行研究的主要领域，特别强调技术问题和具有临床相关性的现有数据。
