Ehara Tatsuya, Kitajima Seiji, Kanzawa Nobuyuki, Tamiya Toru, Tsuchiya Takahide
Department of Chemistry, Faculty of Science and Technology, Sophia University, 102-8554, Tokyo, Japan.
FEBS Lett. 2002 Nov 20;531(3):509-12. doi: 10.1016/s0014-5793(02)03608-6.
Achacin is an antibacterial glycoprotein purified from the mucus of the giant snail, Achatina fulica Férussac, as a humoral defense factor. We showed that achacin has L-amino acid oxidase activity and can generate cytotoxic H(2)O(2); however, the concentration of H(2)O(2) was not sufficient to kill bacteria. The antibacterial activity of achacin was inhibited by various H(2)O(2) scavengers. Immunochemical analysis revealed that achacin was preferentially bound to growth-phase bacteria, accounting for the important role in growth-phase-dependent antibacterial activity of achacin. Achacin may act as an important defense molecule against invading bacteria.
阿奇辛是一种从非洲大蜗牛（Achatina fulica Férussac）黏液中纯化得到的抗菌糖蛋白，作为一种体液防御因子。我们发现阿奇辛具有L-氨基酸氧化酶活性，能够产生具有细胞毒性的过氧化氢；然而，过氧化氢的浓度不足以杀死细菌。阿奇辛的抗菌活性受到多种过氧化氢清除剂的抑制。免疫化学分析表明，阿奇辛优先结合处于生长阶段的细菌，这解释了阿奇辛在依赖生长阶段的抗菌活性中所起的重要作用。阿奇辛可能作为一种抵御入侵细菌的重要防御分子。
