日本人群中A组杀伤细胞免疫球蛋白样受体（KIR）单倍型的优势与KIR表达的多样自然杀伤（NK）细胞库相关。

Predominance of group A KIR haplotypes in Japanese associated with diverse NK cell repertoires of KIR expression.

作者信息

Yawata Makoto, Yawata Nobuyo, McQueen Karina L, Cheng Nathalie W, Guethlein Lisbeth A, Rajalingam Raja, Shilling Heather G, Parham Peter

机构信息

Department of Structural Biology, Stanford University, School of Medicine, 299 Campus Drive West, Sherman Fairchild Building, Stanford, CA 94305-5126, USA.

出版信息

Immunogenetics. 2002 Nov;54(8):543-50. doi: 10.1007/s00251-002-0497-x. Epub 2002 Oct 24.

DOI:10.1007/s00251-002-0497-x

PMID:12439616

Abstract

Genomic DNA from a panel of 41 healthy unrelated Japanese individuals was typed for the presence or absence of 16 KIR genes and pseudogenes. Only eight different KIR genotypes were found. Group A haplotypes outnumbered group B haplotypes in frequency by approximately 3:1, with individuals having two group A haplotypes accounting for 56% of the panel. The frequency of A haplotypes in the Japanese is higher than that observed in other populations. Flow cytometric comparison of KIR expression in 19 panel members showed considerable diversity in NK cell repertoire, which was also seen within the group of individuals having two A haplotypes. This diversity is likely due to allelic polymorphism in expressed genes of the A haplotype. In comparison to other populations, the Japanese appear less heterogeneous in KIR genotype as assessed by gene content.

摘要

对41名健康、无亲缘关系的日本个体的基因组DNA进行了16种杀伤细胞免疫球蛋白样受体（KIR）基因及假基因存在与否的分型。仅发现了8种不同的KIR基因型。A组单倍型在频率上比B组单倍型多约3:1，拥有两个A组单倍型的个体占样本的56%。日本人中A单倍型的频率高于其他人群中观察到的频率。对19名样本成员的KIR表达进行流式细胞术比较显示，自然杀伤（NK）细胞库存在相当大的多样性，在拥有两个A单倍型的个体组中也可见到这种情况。这种多样性可能是由于A单倍型表达基因中的等位基因多态性。与其他人群相比，根据基因含量评估，日本人的KIR基因型似乎异质性较小。