Matsumoto N, Yoshizawa H, Kagamu H, Abe T, Fujita N, Watanabe S, Kuriyama H, Ishiguro T, Tanaka J, Suzuki E, Kobayashi K, Gemma A, Kudoh S, Gejyo F
Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Bone Marrow Transplant. 2002 Dec;30(11):777-84. doi: 10.1038/sj.bmt.1703692.
Although non-frozen storage of peripheral blood stem cells (PBSC) has been extensively studied and utilized clinically, the optimal storage conditions have not been determined. In order to improve the maintenance of clonogenic capacity during storage, we evaluated the feasibility of subzero non-freezing preservation of PBSC and attempted to determine the optimal conditions. Human PBSC were stored in different non-cryopreserved conditions. University of Wisconsin (UW) solution was used as the storage medium for PBSC. The stem cell integrity was optimally maintained when PBSC were preserved in a supercooled state at -2 degrees C in UW solution without any cryoprotectants, and the highest values for nucleated cell survival (91.6%), CFU-GM survival (67.3%) and trypan blue viability (92%) were achieved at 72 h. CFU-GM survival in our storage conditions was significantly better than the survival achieved with hypothermic preservation in autologous serum and ACD-A solution at 4 degrees C (67.3 +/- 9.2% vs 42.9 +/- 15.3%; P < 0.01) or cryopreservation at -80 degrees C (67.3 +/- 9.2% vs 52.7 +/- 10.7%; P < 0.01). Thus, the combination of supercooling and UW solution was the optimal non-freezing method of preserving transplantable PBSC tested here. This method is of clinical utility in peripheral blood stem cell transplantation (PBSCT) for its simplicity and storage efficiency, and has value as a short-term storage method for PBSC to support dose-intensive multicyclic chemotherapy.
尽管外周血干细胞（PBSC）的非冷冻保存已得到广泛研究并在临床上得到应用，但最佳保存条件尚未确定。为了提高保存期间克隆形成能力的维持，我们评估了PBSC亚零非冷冻保存的可行性，并试图确定最佳条件。人PBSC在不同的非冷冻条件下保存。威斯康星大学（UW）溶液用作PBSC的保存介质。当PBSC在-2℃的过冷状态下于不含任何冷冻保护剂的UW溶液中保存时，干细胞完整性得到最佳维持，并且在72小时时获得了有核细胞存活率（91.6%）、CFU-GM存活率（67.3%）和台盼蓝活力（92%）的最高值。在我们的保存条件下CFU-GM存活率显著优于在4℃的自体血清和ACD-A溶液中低温保存（67.3±9.2%对42.9±15.3%；P<0.01）或在-80℃冷冻保存（67.3±9.2%对52.7±10.7%；P<0.01）所获得的存活率。因此，过冷和UW溶液的组合是此处测试的保存可移植PBSC的最佳非冷冻方法。该方法因其简单性和保存效率在外周血干细胞移植（PBSCT）中具有临床实用性，并且作为PBSC的短期保存方法以支持剂量密集型多周期化疗具有价值。
