Weber Wilfried, Kramer Beat P, Fux Cornelia, Keller Bettina, Fussenegger Martin
Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zurich, Switzerland.
J Gene Med. 2002 Nov-Dec;4(6):676-86. doi: 10.1002/jgm.314.
The recently developed heterologous macrolide- (E.REX system) and streptogramin- (PIP system) responsive gene regulation systems show significant differences in their regulation performance in diverse cell lines.
In order to provide optimal regulation modalities for a wide variety of mammalian cell lines, we have performed a detailed analysis of E.REX and PIP systems modified in (i) the transactivation domains of the antibiotic-dependent transactivators, (ii) the type of minimal promoter used, and (iii) the spacing between the operator module and the minimal promoter.
These novel E.REX and PIP regulation components showed not only dramatically improved regulation performance in some cell types, but also enabled their use in cell lines which had previously been inaccessible to regulated transgene expression.
Due to their modular set-up the novel E.REX and PIP regulation systems presented here are most versatile and ready for future upgrades using different cell-specific key regulation components.
最近开发的异源大环内酯类（E.REX系统）和链阳菌素类（PIP系统）响应基因调控系统在不同细胞系中的调控性能存在显著差异。
为了为多种哺乳动物细胞系提供最佳调控模式，我们对E.REX和PIP系统进行了详细分析，这些系统在以下方面进行了修改：（i）抗生素依赖性反式激活因子的反式激活结构域；（ii）所用最小启动子的类型；（iii）操纵子模块与最小启动子之间的间距。
这些新型E.REX和PIP调控组件不仅在某些细胞类型中显著提高了调控性能，还使得它们能够用于以前无法进行转基因表达调控的细胞系。
由于其模块化设置，本文介绍的新型E.REX和PIP调控系统具有很强的通用性，并准备好使用不同的细胞特异性关键调控组件进行未来升级。
