Kirsebom L A
Department of Cell and Molecular Biology, Box 596, Biomedical Center, SE-751 24 Uppsala, Sweden.
Biochem Soc Trans. 2002 Nov;30(Pt 6):1153-8. doi: 10.1042/bst0301153.
The endoribonuclease RNase P is involved in the processing of tRNA precursors to generate mature 5' termini. The catalytic activity of RNase P is associated with an RNA, RNase P RNA. A specific interaction between the 3' end of the substrate and RNase P RNA, to form an RNase P RNA-substrate complex, is referred to as the '73-294-interaction'. This interaction has an important role for efficient and correct cleavage to occur. Here our understanding of the contribution of the 73-294-interaction and metal ions, with respect to efficient and correct cleavage in RNase P RNA-mediated catalysis, will be discussed.
核糖核酸内切酶RNase P参与tRNA前体的加工过程，以生成成熟的5'末端。RNase P的催化活性与一种RNA即RNase P RNA相关。底物3'末端与RNase P RNA之间形成RNase P RNA - 底物复合物的特异性相互作用被称为“73 - 294相互作用”。这种相互作用对于高效且正确的切割的发生具有重要作用。在此，我们将讨论对73 - 294相互作用和金属离子在RNase P RNA介导的催化中对高效且正确切割的贡献的理解。
