通过纳米电喷雾质谱(n)在负离子模式下对未衍生化的中性人乳寡糖进行结构分析(第2部分：应用于异构体混合物)。
Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MS(n) (part 2: application to isomeric mixtures).
作者信息
Pfenninger Anja, Karas Michael, Finke Berndt, Stahl Bend
机构信息
Institute of Pharmaceutical Chemistry, J. W. Goethe University, Frankfurt/Main, Germany.
出版信息
J Am Soc Mass Spectrom. 2002 Nov;13(11):1341-8. doi: 10.1016/S1044-0305(02)00646-3.
A complex mixture of isomeric neutral oligosaccharides from pooled human milk was analyzed by nano-electrospray ionization (ESI) in a quadrupole ion trap mass spectrometer (QIT-MS) in the negative ion mode. Since deprotonated molecules of neutral oligosaccharides follow distinct fragmentation rules, which have been elucidated by using model compounds (see [1]), spectra obtained from consecutive CID experiments allowed the differentiation of isomers out of this highly complex mixture. With this method new human milk oligosaccharides of previously unknown isomeric structures have been identified, e.g., the occurence of three isomeric fucosylated lacto-N-hexaoses could be determined precisely, which have not been described before: (1) Fuc (alpha1-->2) Gal (beta1-->3) GlcNac (beta1-->3) Gal (beta1-->4) GlcNac (beta1-->3) Gal (beta1-->4) Glc, (2) Gal (beta1-->4) GlcNAc [(alpha1-->3) Fuc] (beta1-->3) Gal (beta1-->4) GlcNac (beta1-->3) Gal (beta1-->4) Glc, (3) Gal (beta1-->4) GlcNAc (beta1-->3) Gal (beta1-->4) GlcNac [(alpha1-->3) Fuc] (beta1-->3) Gal (beta1-->4) Glc.
采用负离子模式，在四极杆离子阱质谱仪（QIT-MS）中通过纳米电喷雾电离（ESI）对来自混合人乳的异构中性寡糖的复杂混合物进行了分析。由于中性寡糖的去质子化分子遵循不同的碎片化规则，这些规则已通过使用模型化合物得以阐明（见[1]），连续碰撞诱导解离（CID）实验获得的光谱使得能够从这种高度复杂的混合物中区分异构体。通过这种方法，已鉴定出具有先前未知异构结构的新人乳寡糖，例如，可以精确确定三种异构岩藻糖基化乳糖-N-己糖的存在，此前尚未对其进行过描述：（1）Fuc（α1→2）Gal（β1→3）GlcNac（β1→3）Gal（β1→4）GlcNac（β1→3）Gal（β1→4）Glc，（2）Gal（β1→4）GlcNAc[（α1→3）Fuc]（β1→3）Gal（β1→4）GlcNac（β1→3）Gal（β1→4）Glc，（3）Gal（β1→4）GlcNAc（β1→3）Gal（β1→4）GlcNac[（α1→3）Fuc]（β1→3）Gal（β1→4）Glc。
Zotero 插件