Yang Yinhui, Fu Xiaobing, Li Junyou
Trauma Centre, Burn Department, 304th Hospital of PLA, Beijing 100037, China.
Chin J Traumatol. 2002 Dec;5(6):342-5.
To investigate the proliferative effect of keratinocyte growth factor (KGF-2) on human adult keratinocytes.
The standard medium was keratinocyte growth medium without bovine pituitary extract (BPE), hydrocortisone or epidermal growth factor (EGF). Keratinocytes from a 48-year-old subject were cultured and seeded on dishes with standard medium of EGF in cell density of 2 x 10(4)/32 mm(2). After 24 hours, the medium was replaced by the standard medium with 0, 4, 16, 125 and 500 ng/ml KGF-2, respectively. The standard medium with EGF was used as the positive control and the standard medium without EGF or KGF-2 was used as the negative controls. The growth of keratinocytes was monitored by 3-(4,5-dimethythiazol-2-yl)-2,5 dipheyl tetrazolium bromide (MTT) assay and by photographs on days 3, 5 and 7, respectively.
KGF-2 in concentrations of 4-500 ng/ml showed a significant proliferative effect on days 5 and 7 as compared with that of the negative controls (P < 0.01). On day 3 the cells were proliferated to 1.5-2.5-fold, on day 5 to 3-5-fold and on day 7 to 3-12-fold in KGF-2 medium as that of the negative controls. The optimal response occurred when the concentration of KGF-2 was 125 ng/ml on day 7. Cell proliferation was also consistently higher in all KGF-2 concentrations as compared with that of the positive controls.
KGF-2 has significant effects on the proliferation of adult keratinocytes, which are more effective than that of EGF. This study supports KGF-2 can improve the healing of chronic wounds in adults in clinic.
研究角质形成细胞生长因子（KGF - 2）对成人角质形成细胞的增殖作用。
标准培养基为不含牛垂体提取物（BPE）、氢化可的松或表皮生长因子（EGF）的角质形成细胞生长培养基。将一名48岁受试者的角质形成细胞进行培养，并以2×10⁴/32 mm²的细胞密度接种于含EGF标准培养基的培养皿中。24小时后，分别用含0、4、16、125和500 ng/ml KGF - 2的标准培养基替换原培养基。含EGF的标准培养基用作阳性对照，不含EGF或KGF - 2的标准培养基用作阴性对照。分别通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑（MTT）法以及在第3、5和7天拍照来监测角质形成细胞的生长情况。
与阴性对照相比，浓度为4 - 500 ng/ml的KGF - 2在第5天和第7天显示出显著的增殖作用（P < 0.01）。在第3天，KGF - 2培养基中的细胞增殖至阴性对照的1.5 - 2.5倍，第5天为3 - 5倍，第7天为3 - 12倍。第7天KGF - 2浓度为125 ng/ml时出现最佳反应。与阳性对照相比，所有KGF - 2浓度下的细胞增殖也始终更高。
KGF - 2对成人角质形成细胞的增殖有显著作用，且比EGF更有效。本研究支持KGF - 2在临床上可促进成人慢性伤口的愈合。
