Ozkurt Z, Erol S, Tasyaran M A, Kaya A
Department of Clinic Bacteriology and Infectious Diseases, Atatürk University Medical Faculty, Mersin, Turkey.
Clin Microbiol Infect. 2002 Nov;8(11):749-52. doi: 10.1046/j.1469-0691.2002.00471.x.
This study was conducted to evaluate the ability of the BacT/Alert automated blood culture system to detect Brucella spp. in comparison with traditional Brucella broth culture. Overall, 100 (50 bone marrow and 50 blood samples) paired cultures were obtained, and 59 were positive by at least one method. The Brucella broth culture method detected all 59 positive cultures (100%), and the BacT/Alert system detected 30 (50.8%) (P < 0.05). The mean detection times for B. melitensis were 4.5 days in the BacT/Alert system and 5 days in Brucella broth culture (P > 0.05). There is no significant difference between the two methods with respect to growth time of the microorganism, but Brucella broth culture is more sensitive than the BacT/Alert system.
本研究旨在评估BacT/Alert自动血培养系统与传统布鲁氏菌肉汤培养法相比检测布鲁氏菌属的能力。总体而言，共获得100对培养物（50份骨髓样本和50份血液样本），其中59份通过至少一种方法检测为阳性。布鲁氏菌肉汤培养法检测出所有59份阳性培养物（100%），而BacT/Alert系统检测出30份（50.8%）（P<0.05）。在BacT/Alert系统中，羊种布鲁氏菌的平均检测时间为4.5天，在布鲁氏菌肉汤培养中为5天（P>0.05）。两种方法在微生物生长时间方面无显著差异，但布鲁氏菌肉汤培养比BacT/Alert系统更敏感。
