Yagupsky P
Clinical Microbiology Laboratory, Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
J Clin Microbiol. 1994 Aug;32(8):1899-901. doi: 10.1128/jcm.32.8.1899-1901.1994.
Data on the performance of modern blood culture systems for the detection of Brucella spp. are insufficient. To evaluate the performance of the BACTEC NR660 blood culture system for the detection of Brucella melitensis within the routine 1-week blood culture protocol, a prospective 24-month study was conducted in an area endemic for B. melitensis in southern Israel. Blood samples obtained from patients with suspected brucellosis were monitored and blindly subcultured once per week for 4 weeks, and the fraction of blood cultures positive for B. melitensis detected by the BACTEC NR660 instrument within the first week was determined. During the study period, a total of 373 blood cultures were obtained from patients in whom brucellosis was suspected, and 27 (7.2%) of them, drawn from 21 different patients, were positive for B. melitensis. Twenty-one (78.8%) of these positive cultures were detected by the BACTEC instrument within 7 days, and six positive cultures were detected by subculture after 2 or 3 weeks of incubation. It is concluded that the BACTEC NR660 blood culture system detects the majority of B. melitensis isolates within the routine 1-week blood culture schedule. To maximize the recovery of the organism, however, prolonged incubation and periodic performance of blind subcultures are still required.
关于现代血培养系统检测布鲁氏菌属性能的数据不足。为评估BACTEC NR660血培养系统在常规1周血培养方案中检测羊种布鲁氏菌的性能，在以色列南部羊种布鲁氏菌流行地区进行了一项为期24个月的前瞻性研究。对疑似布鲁氏菌病患者采集的血样进行监测，并每周盲目传代培养一次，持续4周，同时确定BACTEC NR660仪器在第一周内检测到的羊种布鲁氏菌血培养阳性率。在研究期间，共从疑似布鲁氏菌病患者中采集了373份血培养样本，其中27份（7.2%）来自21名不同患者的样本羊种布鲁氏菌呈阳性。这些阳性培养物中有21份（78.8%）在7天内被BACTEC仪器检测到，6份阳性培养物在培养2或3周后通过传代培养检测到。结论是，BACTEC NR660血培养系统在常规1周血培养方案中能检测到大多数羊种布鲁氏菌分离株。然而，为了最大限度地回收该病原体，仍需要延长培养时间并定期进行盲目传代培养。
