活细胞中天然启动子上转录因子的动态行为
Dynamic behavior of transcription factors on a natural promoter in living cells.
作者信息
Becker Matthias, Baumann Christopher, John Sam, Walker Dawn A, Vigneron Marc, McNally James G, Hager Gordon L
机构信息
Laboratory of Receptor Biology and Gene Expression, Building 41 Room B602, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-5055, USA.
出版信息
EMBO Rep. 2002 Dec;3(12):1188-94. doi: 10.1093/embo-reports/kvf244. Epub 2002 Nov 21.
Abstract
Through the use of photobleaching techniques, we examined the dynamic interaction of three members of the transcription apparatus with a target promoter in living cells. The glucocorticoid receptor (GR) interacting protein 1 (GRIP-1) exhibits a half maximal time for fluorescent recovery (tau(R)) of 5 s, reflecting the same rapid exchange as observed for GR. In contrast, the large subunit (RPB1) of RNA polymerase II (pol II) required 13 min for complete fluorescence recovery, consistent with its function as a processive enzyme. We also observe a complex induction profile for the kinetics of GR-stimulated transcription. Our results indicate that GR and GRIP-1 as components of the activating complex are in a dynamic equilibrium with the promoter, and must return to the template many times during the course of transcriptional activation.
摘要
通过使用光漂白技术，我们研究了转录装置的三个成员与活细胞中目标启动子的动态相互作用。糖皮质激素受体（GR）相互作用蛋白1（GRIP - 1）的荧光恢复半衰期（tau(R)）为5秒，这反映出与GR相同的快速交换。相比之下，RNA聚合酶II（pol II）的大亚基（RPB1）需要13分钟才能完全恢复荧光，这与其作为持续性酶的功能一致。我们还观察到GR刺激转录动力学的复杂诱导模式。我们的结果表明，作为激活复合物组分的GR和GRIP - 1与启动子处于动态平衡，并且在转录激活过程中必须多次返回模板。
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本文引用的文献
1
Combinatorial control of gene expression by nuclear receptors and coregulators.核受体与共调节因子对基因表达的组合调控。
Cell. 2002 Feb 22;108(4):465-74. doi: 10.1016/s0092-8674(02)00641-4.
2
The RNA polymerase II machinery: structure illuminates function.RNA聚合酶II机制：结构揭示功能。
Cell. 2002 Feb 22;108(4):453-63. doi: 10.1016/s0092-8674(02)00646-3.
3
Protein dynamics in the nuclear compartment.细胞核内的蛋白质动力学
Curr Opin Genet Dev. 2002 Apr;12(2):137-41. doi: 10.1016/s0959-437x(02)00278-2.
4
Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter.由天然启动子转录调控的大规模染色质解聚和再凝聚
J Cell Biol. 2001 Jul 9;154(1):33-48. doi: 10.1083/jcb.200011069.
5
Steroid hormone receptor-mediated histone deacetylation and transcription at the mouse mammary tumor virus promoter.类固醇激素受体介导的小鼠乳腺肿瘤病毒启动子处的组蛋白去乙酰化与转录
J Biol Chem. 2001 Aug 31;276(35):32423-6. doi: 10.1074/jbc.C100315200. Epub 2001 Jul 11.
6
Ligand-mediated assembly and real-time cellular dynamics of estrogen receptor alpha-coactivator complexes in living cells.配体介导的雌激素受体α-共激活因子复合物在活细胞中的组装及实时细胞动力学
Mol Cell Biol. 2001 Jul;21(13):4404-12. doi: 10.1128/MCB.21.13.4404-4412.2001.
7
The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome.糖皮质激素受体相互作用蛋白1（GRIP1）定位于与ND10小体相关的离散核灶中，且富含26S蛋白酶体的成分。
Mol Endocrinol. 2001 Apr;15(4):485-500. doi: 10.1210/mend.15.4.0618.
8
Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.雌激素受体调控转录中的辅助因子动力学与充足性
Cell. 2000 Dec 8;103(6):843-52. doi: 10.1016/s0092-8674(00)00188-4.
9
Mammalian cell lines expressing functional RNA polymerase II tagged with the green fluorescent protein.表达与绿色荧光蛋白标记的功能性RNA聚合酶II的哺乳动物细胞系。
J Cell Sci. 2000 Aug;113 ( Pt 15):2679-83. doi: 10.1242/jcs.113.15.2679.
10
The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.糖皮质激素受体：与活细胞中调节位点的快速交换。
Science. 2000 Feb 18;287(5456):1262-5. doi: 10.1126/science.287.5456.1262.
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