Gonzalez-Benito M Elena, Prieto Roberto-Moreno, Herradon Esther, Martin Carmen
Departamento de Biologia Vegetal, Escuela Universitaria de Ingenieria Tecnica Agricola, Universidad Politecnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain.
Cryo Letters. 2002 Sep-Oct;23(5):283-90.
This study examines different factors included in the cryopreservation protocols for Quercus ilex and Q. suber embryonic axes. In vitro incubation temperature played an important role in the appropriate development of Q. ilex axes, as 15 degrees C was superior to 25 degrees C. Q. suber axes proved to be more sensitive to desiccation and cooling. Poor survival (35%) was observed when axes were included into cryovials and then in liquid nitrogen, and none when immersed in sub-cooled liquid nitrogen (-210 degrees C). Q. ilex axes showed poorly organised development in vitro (c. 50% of non-cooled axes showed shoot development). However, c. 80% survival was observed after cryopreservation (either in liquid nitrogen or sub-cooled liquid nitrogen at 0.34 g water / g dry weight), of which c. 15% showed shoot development.
本研究考察了冬青栎和栓皮栎胚轴冷冻保存方案中的不同因素。体外培养温度对冬青栎胚轴的正常发育起着重要作用，因为15℃优于25℃。栓皮栎胚轴对干燥和冷却更为敏感。当胚轴放入冻存管然后置于液氮中时，存活率较低（35%），而浸入过冷液氮（-210℃）中时则无一存活。冬青栎胚轴在体外发育不良（约50%未冷却的胚轴有芽发育）。然而，冷冻保存后（在液氮或0.34 g水/g干重的过冷液氮中）观察到约80%的存活率，其中约15%有芽发育。
