Gubareva Larisa V, Novikov Dmitri V, Hayden Frederick G
Department of Internal Medicine, University of Virginia School of Medicine Health Sciences System, Charlottesville 22908, USA.
J Infect Dis. 2002 Dec 1;186(11):1575-81. doi: 10.1086/345372. Epub 2002 Nov 11.
To examine the molecular epidemiology of influenza virus transmission, the nucleotide sequences of the HA1 domain of the hemagglutinin (HA) gene of 57 influenza A and 24 influenza B viruses recovered in a single season were analyzed. No nucleotide sequence differences were found among the 10 viruses that were recovered twice from the same patient. The nucleotide sequences of influenza A viruses were identical within each family but varied among the 14 families included in the study. The sequences of influenza A viruses recovered from 18 residents of the same community showed that 83% of the viruses differed from the others by at least 1 nucleotide residue. These findings indicate that most cases of influenza in households in which 1 family member has been infected are the result of secondary transmission from the index patient and not of acquisition from other community sources. Substantial genetic conservation during virus transmission within households is indicated.
为研究流感病毒传播的分子流行病学，对在单一季节中分离出的57株甲型流感病毒和24株乙型流感病毒血凝素（HA）基因的HA1结构域核苷酸序列进行了分析。在从同一患者体内两次分离出的10株病毒中未发现核苷酸序列差异。甲型流感病毒的核苷酸序列在每个家族内是相同的，但在所研究的14个家族之间存在差异。从同一社区的18名居民中分离出的甲型流感病毒序列显示，83%的病毒与其他病毒至少有1个核苷酸残基的差异。这些发现表明，在有一名家庭成员感染的家庭中，大多数流感病例是由首例患者继发传播所致，而非从社区其他来源感染。这表明在家庭内病毒传播过程中存在显著的基因保守性。
