State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
J Clin Virol. 2011 Oct;52(2):146-50. doi: 10.1016/j.jcv.2011.06.022. Epub 2011 Jul 29.
There are few data in the literature on viral sequence variation between host generations/successive transmission events. Relatively little is known about the sequence heterogeneity of the influenza viruses transmitted within families.
To study the molecular epidemiology of influenza virus and to determine the sequence variation within an individual, a household and a community during the first wave of influenza pandemic in 2009.
A prospective study of household transmission of influenza A in Hong Kong was conducted during the pandemic in 2009. The HA and NA sequences of pandemic and seasonal influenza A viral isolates identified in this household transmission study were sequences and analyzed.
Our results indicated that there were multiple introductions of influenza viruses into Hong Kong. Sequence analysis of these isolates suggested that members of these family clusters acquired the infection by household transmissions. Interestingly, unlike those concluded from previous household transmission studies, we observed sequence variations between sequential samples from the same person and also within the same household.
Family clusters of influenza A viral infection are predominantly the result of secondary transmission within a household. Our results also suggested that the intra-host viral sequence variation might be more common that than previously thought.
关于宿主代际/连续传播事件之间病毒序列变异的文献数据很少。对于家庭内传播的流感病毒的序列异质性，人们知之甚少。
研究流感病毒的分子流行病学，并确定 2009 年流感大流行期间个体、家庭和社区内的序列变异。
在 2009 年流感大流行期间，对香港的家庭传播流感 A 进行了前瞻性研究。对该家庭传播研究中鉴定的大流行和季节性流感 A 病毒分离株的 HA 和 NA 序列进行了分析。
我们的结果表明，有多种流感病毒传入香港。对这些分离株的序列分析表明，这些家族群的成员是通过家庭传播感染的。有趣的是，与之前的家庭传播研究得出的结论不同，我们观察到来自同一人的连续样本之间以及同一家庭内的序列变化。
流感 A 病毒感染的家族群主要是家庭内二次传播的结果。我们的研究结果还表明，宿主内病毒序列变异可能比以前认为的更为常见。
