Miggiano V C, Meo T, Bergén I, Nabholz M
Tissue Antigens. 1975 Apr;5(3):173-85. doi: 10.1111/j.1399-0039.1975.tb01212.x.
A microtechnique (10 mul culture volume) for in vitro lymphocyte cultures is described, which, compared with current miniaturized techniques, permits a four to 20-fold reduction of both medium volume and cell numbers. Furthermore, two alternative procedures for harvesting and washing radioisotope labelled cells are described. The first, making use of a conventional harvesting device, collects cells on glass fibre filters in a two to three-fold shorter time than that needed for the current semi-automatic collectors. The second takes advantage of the possibility of washing and fixing the cells in their culture wells and allows processing of more cultures with fewer manual operations. Various technical aspects of the micro-culture system are described and discussed with special reference to automation problems.
本文描述了一种用于体外淋巴细胞培养的微量技术（培养体积为10微升），与目前的小型化技术相比，该技术可使培养基体积和细胞数量减少4至20倍。此外，还描述了两种用于收获和洗涤放射性同位素标记细胞的替代方法。第一种方法利用传统的收获装置，在玻璃纤维滤器上收集细胞，所需时间比目前的半自动收集器短两到三倍。第二种方法利用在培养孔中洗涤和固定细胞的可能性，并且通过较少的手动操作即可处理更多的培养物。本文描述并讨论了微培养系统的各个技术方面，并特别提及了自动化问题。
