Rella W
J Immunol Methods. 1978;21(3-4):237-45. doi: 10.1016/0022-1759(78)90150-3.
Experiments were conducted to standardize the response of lymphocytes in whole blood in mixed culture with allogeneic lymphocytes. The following conditions were found suitable: (1) A culture period of 6-8 days. (2) The ratio of stimulator to responder lymphocytes should be 4-8, but may vary from one batch of stimulator cells to another. (3) Tests may be performed in culture tubes with 50-100 microliter blood in a total volume of 2 ml (macrotechnique) or in microplates with 10 microliter of blood in a volume of 0.2 ml (microtechnique). (4) Serum supplement is not required. (5) Results should be expressed as counts/min (cpm) per a given number of responder lymphocytes. Stimulation indices are less reliable.
进行实验以标准化全血中淋巴细胞与同种异体淋巴细胞混合培养的反应。发现以下条件合适：(1)培养期为6 - 8天。(2)刺激细胞与反应细胞淋巴细胞的比例应为4 - 8，但不同批次的刺激细胞可能有所不同。(3)测试可在含有50 - 100微升血液、总体积为2毫升的培养管中进行（宏观技术），或在含有10微升血液、体积为0.2毫升的微孔板中进行（微观技术）。(4)不需要血清补充。(5)结果应以每给定数量的反应细胞淋巴细胞的计数/分钟（cpm）表示。刺激指数不太可靠。
