Stewart T C
Clin Chem. 1976 Jan;22(1):74-8.
The hexokinase method (I) for glucose in biological fluids, proposed by the FDA as a potential national standard method, is compared with the Eyetone/Dextrostix/Control method (II) for measurement of glucose in whole blood. I used a new, two-step calibration procedure based on a 1.30 g/liter standard for the II system. The correlation coefficient for results by the two methods was 0.99 for 142 assays in duplicate. Recovery values were near 100% (I, range 100.0 to 106.2%; II, range 100.8 to 105.1%), and the coefficients of variation were respectively +/- 4.1% and +/- 4.5%. Considering the close correlation between the two methods, together with the simplicity, speed, and convenience of II, I conclude that it has great clinical utility, particularly in emergency situations. The precision and accuracy of II depends greatly on proper calibration and control procedures and on adequate training of the operator.
美国食品药品监督管理局（FDA）提议作为潜在国家标准方法的生物体液中葡萄糖的己糖激酶法（I），与全血葡萄糖测定的Eyetone/Dextrostix/对照法（II）进行了比较。对于II系统，我使用了基于1.30克/升标准品的新的两步校准程序。两种方法的结果的相关系数在142次重复测定中为0.99。回收率接近100%（I，范围为100.0%至106.2%；II，范围为100.8%至105.1%），变异系数分别为±4.1%和±4.5%。考虑到两种方法之间的密切相关性，以及II的简单性、速度和便利性，我得出结论，它具有很大的临床实用性，特别是在紧急情况下。II的精密度和准确性在很大程度上取决于正确的校准和控制程序以及操作人员的充分培训。
