Perbellini Luigi, Veronese Nello, Princivalle Andrea
Medicina del Lavoro, Department of Medicine and Public Health, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Dec 5;781(1-2):269-90. doi: 10.1016/s1570-0232(02)00501-9.
This paper reviews several procedures for determination of mercapturic acids in urine. Special attention was paid to methods useful in relation to human exposure to industrial pollutants, without any description for less sensitive methods used in animal research. Gas chromatographic and liquid chromatographic procedures were considered together with the little information available about thin layer chromatography and immunochemical techniques. After a description of the main industrial pollutants which lead to synthesis of their specific mercapturic acids, the methods for analysing these products are synthetically reported. The comparison among difficulties in sample preparation, complexity of instrumentation and their cost/benefit ratio are discussed.
