Matsui Masanori, Moriya Osamu, Abdel-Aziz Nada, Matsuura Yoshiharu, Miyamura Tatsuo, Akatsuka Toshitaka
Department of Microbiology, Saitama Medical School, Moroyama-Cho, Iruma-Gun, Saitama 350-0495, Japan.
Vaccine. 2002 Dec 13;21(3-4):211-20. doi: 10.1016/s0264-410x(02)00460-7.
We studied the potential of dendritic cells (DCs) in priming hepatitis C virus (HCV)-specific cytotoxic T lymphocytes (CTLs) in mice. Recombinant adenovirus expressing HCV core (Adex1SR3ST) was employed to express core in DCs. Core-specific CTLs are effectively elicited by injecting Adex1SR3ST-transduced DCs, whereas injection of Adex1SR3ST does not result in effective priming. Further, Adex1SR3ST-transduced DCs more efficiently prime core-specific CTLs than Adex1SR3ST-transduced macrophages, or DCs treated with an anthrax toxin fusion protein reported previously. Upon challenge with recombinant HCV-core-expressing vaccinia virus, vaccinia titers are significantly reduced in mice immunized with Adex1SR3ST-transduced DCs. Thus, adenovirus-transduced DCs may be a promising candidate for a CTL-based vaccine against HCV.
我们研究了树突状细胞（DCs）在小鼠体内启动丙型肝炎病毒（HCV）特异性细胞毒性T淋巴细胞（CTLs）的潜力。利用表达HCV核心蛋白的重组腺病毒（Adex1SR3ST）在DCs中表达核心蛋白。通过注射Adex1SR3ST转导的DCs可有效诱导核心特异性CTLs，而注射Adex1SR3ST则不能有效启动免疫反应。此外，与Adex1SR3ST转导的巨噬细胞或先前报道的用炭疽毒素融合蛋白处理的DCs相比，Adex1SR3ST转导的DCs能更有效地启动核心特异性CTLs。在用表达重组HCV核心蛋白的痘苗病毒攻击后，用Adex1SR3ST转导的DCs免疫的小鼠体内痘苗病毒滴度显著降低。因此，腺病毒转导的DCs可能是基于CTL的抗HCV疫苗的有前途的候选者。
