Matsui Masanori, Moriya Osamu, Akatsuka Toshitaka
Department of Microbiology, Saitama Medical School, Moroyama-Cho, Iruma-Gun, 350-0495, Saitama, Japan.
Vaccine. 2003 Apr 2;21(15):1629-39. doi: 10.1016/s0264-410x(02)00704-1.
We evaluated the prime-boost immunization consisting of hepatitis C virus (HCV)-core expression plasmid (pCEP4-core) and replication-defective adenovirus expressing HCV-core (Adex1SR3ST) for core-specific CTL induction in mice. Compared to a single booster, double boosters after priming enhance CTL induction. The prime-double boosts immunization involving pCEP4-core priming followed by pCEP4-core and Adex1SR3ST boostings (pC/pC/aC) can induce core-specific CTLs as well as other combinations: pC/aC/aC; aC/pC/pC; aC/aC/aC, whereas pC/pC/pC does not induce CTLs. Furthermore, co-administration of interleukin-12 (IL-12) expression plasmid leads to the highly efficient CTL induction and clearance of HCV-core expressing vaccinia virus challenged. Thus, the prime-double boosts immunization together with IL-12 may be promising for HCV vaccine.
我们评估了由丙型肝炎病毒（HCV）核心表达质粒（pCEP4-core）和表达HCV核心的复制缺陷型腺病毒（Adex1SR3ST）组成的初免-加强免疫方案在小鼠中诱导核心特异性CTL的效果。与单次加强免疫相比，初免后进行两次加强免疫可增强CTL的诱导。涉及pCEP4-core初免，随后进行pCEP4-core和Adex1SR3ST加强免疫（pC/pC/aC）的初免-两次加强免疫方案能够诱导核心特异性CTL，其他组合方案：pC/aC/aC；aC/pC/pC；aC/aC/aC也能诱导，而pC/pC/pC则不能诱导CTL。此外，白细胞介素-12（IL-12）表达质粒的共同给药可导致高效的CTL诱导，并清除受痘苗病毒攻击后表达HCV核心的病毒。因此，初免-两次加强免疫方案联合IL-12对于HCV疫苗可能是有前景的。
