球形芽孢杆菌中真正磷酸果糖激酶的存在：pfk基因的克隆与测序
Existence of a true phosphofructokinase in Bacillus sphaericus: cloning and sequencing of the pfk gene.
作者信息
Alice Alejandro F, Pérez-Martínez Gaspar, Sánchez-Rivas Carmen
机构信息
Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
出版信息
Appl Environ Microbiol. 2002 Dec;68(12):6410-5. doi: 10.1128/AEM.68.12.6410-6415.2002.
Abstract
Some strains of Bacillus sphaericus are entomopathogenic to mosquito larvae, which transmit diseases, such as filariasis and malaria, affecting millions of people worldwide. This species is unable to use hexoses and pentoses as unique carbon sources, which was proposed to be due to the lack of glycolytic enzymes, such as 6-phosphofructokinase (PFK). In this study, PFK activity was detected and the pfk gene was cloned and sequenced. Furthermore, this gene was shown to be present in strains belonging to all the homology groups of this heterogeneous species, in which PFK activity was also detected. A careful sequence analysis revealed the conservation of different catalytic and regulatory residues, as well as the enzyme's phylogenetic affiliation with the family of allosteric ATP-PFK enzymes.
摘要
一些球形芽孢杆菌菌株对蚊子幼虫具有昆虫致病性，蚊子幼虫会传播疾病，如丝虫病和疟疾，影响着全球数百万人。该物种无法将己糖和戊糖作为唯一碳源利用，据推测这是由于缺乏糖酵解酶，如6-磷酸果糖激酶（PFK）。在本研究中，检测到了PFK活性，并对pfk基因进行了克隆和测序。此外，该基因存在于属于这个异质物种所有同源组的菌株中，在这些菌株中也检测到了PFK活性。仔细的序列分析揭示了不同催化和调节残基的保守性，以及该酶与变构ATP-PFK酶家族的系统发育关系。
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