Ventura Marco, Zink Ralf
Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland.
Appl Environ Microbiol. 2002 Dec;68(12):6429-34. doi: 10.1128/AEM.68.12.6429-6434.2002.
Identification of Bifidobacterium lactis and Bifidobacterium animalis is problematic because of phenotypic and genetic homogeneities and has raised the question of whether they belong to one unique taxon. Analysis of the 16S-23S internally transcribed spacer region of B. lactis DSM10140(T), B. animalis ATCC 25527(T), and six potential B. lactis strains suggested two distinct clusters. Two specific 16S-23S spacer rRNA gene-targeted primers have been developed for specific detection of B. animalis. All of the molecular techniques used (B. lactis or B. animalis PCR primers, enterobacterial repetitive intergenic consensus PCR) demonstrated that B. lactis and B. animalis form two main groups and suggest a revision of the strains assigned to B. animalis. We propose that B. lactis should be separated from B. animalis at the subspecies level.
由于表型和基因的同质性，乳酸双歧杆菌和动物双歧杆菌的鉴定存在问题，这引发了它们是否属于一个独特分类单元的问题。对乳酸双歧杆菌DSM10140(T)、动物双歧杆菌ATCC 25527(T)以及六个潜在的乳酸双歧杆菌菌株的16S-23S内转录间隔区进行分析，结果显示出两个不同的聚类。已开发出两种针对16S-23S间隔区rRNA基因的特异性引物，用于动物双歧杆菌的特异性检测。所使用的所有分子技术（乳酸双歧杆菌或动物双歧杆菌PCR引物、肠杆菌重复基因间共有序列PCR）均表明，乳酸双歧杆菌和动物双歧杆菌形成两个主要类群，并建议对归为动物双歧杆菌的菌株进行修订。我们建议在亚种水平上，将乳酸双歧杆菌与动物双歧杆菌区分开来。
