Bakonyi Tamás, Grabensteiner Elvira, Kolodziejek Jolanta, Rusvai Miklós, Topolska Grazyna, Ritter Wolfgang, Nowotny Norbert
Department of Microbiology and Infectious Diseases, Faculty of Veterinary Sciences, Szent István University, H-1143 Budapest, Hungary.
Appl Environ Microbiol. 2002 Dec;68(12):6446-50. doi: 10.1128/AEM.68.12.6446-6450.2002.
Reverse transcription-PCR assays have been established for a quick, sensitive, and specific diagnosis of acute bee paralysis virus (ABPV), a common virus of the honeybee (Apis mellifera), directly from clinical samples. A 3,071-nucleotide fragment of the ABPV genome, which includes the entire capsid polyprotein gene, was amplified from Austrian, German, Polish, and Hungarian ABPV samples and sequenced, and the sequences were compared. The alignment of a smaller fragment with ABPV sequences from the United States and the United Kingdom revealed nucleotide identity rates between 89 and 96%, respectively. Phylogenetic trees which display the molecular relationship between the viruses of different geographic origin were constructed.
已建立逆转录聚合酶链反应(RT-PCR)检测方法,可直接从临床样本中快速、灵敏且特异地诊断急性蜜蜂麻痹病毒(ABPV),这是蜜蜂(西方蜜蜂)的一种常见病毒。从奥地利、德国、波兰和匈牙利的ABPV样本中扩增出包含整个衣壳多聚蛋白基因的3071个核苷酸的ABPV基因组片段并进行测序,然后对序列进行比较。将一个较小片段与来自美国和英国的ABPV序列进行比对,发现核苷酸同一性率分别在89%至96%之间。构建了显示不同地理来源病毒之间分子关系的系统发育树。