使用特异性DNA探针通过菌落杂交法对嗜肺军团菌菌落形成单位进行计数。
Enumeration of Legionella CFU by colony hybridization using specific DNA probes.
作者信息
Satoh Wakako, Nakata Mayumi, Yamamoto Hiroyuki, Ezaki Takayuki, Hiramatsu Keiichi
机构信息
Technology and Development Laboratory, Kurita Water Industries Ltd., Kanagawa 243-0124, Japan.
出版信息
Appl Environ Microbiol. 2002 Dec;68(12):6466-70. doi: 10.1128/AEM.68.12.6466-6470.2002.
Abstract
Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.
摘要
应用寡核苷酸探针和菌落杂交（CH）技术对冷却塔水中嗜肺军团菌属微生物进行计数。CH计数结果与源自该水样的培养样本中的菌落形成单位（CFU）计数结果几乎相同。得出的结论是，CH方法有可能替代传统方法。
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