Kay Sarina, Thapa Anil, Haberstroh Karen M, Webster Thomas J
Deparment of Biomedical Engineering, Purdue University, 1296 Potter Building, West Lafayette, IN 49707, USA.
Tissue Eng. 2002 Oct;8(5):753-61. doi: 10.1089/10763270260424114.
Osteoblast (bone-forming cell) and chondrocyte (cartilage-synthesizing cell) adhesion on novel nanostructured polylactic/glycolic acid (PLGA) and titania composites were investigated in the present in vitro study. Nanostructured polymers were created by chemically treating micron-structured PLGA with select concentrations of NaOH for various periods of time. Dimensions of ceramics were controlled by utilizing either micron or nanometer grain size titania. Compared with surfaces with conventional or micron surface roughness dimensions, results provided the first evidence of increased osteoblast and chondrocyte adhesion on 100 wt% PLGA films with nanometer polymer surface roughness dimensions. Results also confirmed other literature reports of enhanced osteoblast adhesion on 100 wt% nanometer compared with conventional grain size titania compacts; however, the present study provided the first evidence that decreasing titania grain size into the nanometer range did not influence chondrocyte adhesion. Finally, osteoblast and chondrocyte adhesion increased on 70/30 wt% PLGA/titania composites formulated to possess nanosurface rather than conventional surface feature dimensions. The present study, thus, provided evidence that these nanostructured PLGA/titania composites may possess the ability to simulate surface and/or chemical properties of bone and cartilage, respectively, to allow for exciting alternatives in the design of prostheses with greater efficacy.
在本体外研究中，对成骨细胞（骨形成细胞）和软骨细胞（软骨合成细胞）在新型纳米结构聚乳酸/乙醇酸共聚物（PLGA）和二氧化钛复合材料上的黏附情况进行了研究。通过用特定浓度的氢氧化钠在不同时间段对微米结构的PLGA进行化学处理来制备纳米结构聚合物。利用微米或纳米粒度的二氧化钛来控制陶瓷的尺寸。与具有常规或微米表面粗糙度尺寸的表面相比，结果首次证明了在具有纳米聚合物表面粗糙度尺寸的100 wt% PLGA薄膜上，成骨细胞和软骨细胞的黏附有所增加。结果还证实了其他文献报道，即与常规粒度的二氧化钛压块相比，100 wt%纳米级二氧化钛上的成骨细胞黏附增强；然而，本研究首次证明将二氧化钛粒度减小到纳米范围不会影响软骨细胞的黏附。最后，在配制为具有纳米表面而非常规表面特征尺寸的70/30 wt% PLGA/二氧化钛复合材料上，成骨细胞和软骨细胞的黏附增加。因此，本研究提供了证据，表明这些纳米结构的PLGA/二氧化钛复合材料可能分别具有模拟骨和软骨的表面和/或化学性质的能力，从而为设计更有效的假体提供令人兴奋的替代方案。
