Savaiano Jennifer K, Webster Thomas J
Department of Biomedical Engineering, Purdue University, 1296 Potter Eng. Building, West Lafayette, IN 47907-1296, USA.
Biomaterials. 2004 Mar-Apr;25(7-8):1205-13. doi: 10.1016/j.biomaterials.2003.08.012.
Chondrocyte (cartilage-synthesizing cells) cell density and synthesis of select intracellular proteins by chondrocytes were investigated on novel nanophase poly-lactic/glycolic acid (PLGA) and titania composites in the present in vitro study. Nanophase PLGA films were created by chemically treating conventional (or micron-structured) PLGA films with 10N NaOH for 1h. Titania particle dimensions in ceramic compacts were controlled by utilizing either conventional (i.e., micron) or nanometer grain size titania. Composites of either conventional or nanophase PLGA with either conventional or nanophase titania at 70/30wt% were also created. Compared to surfaces with a conventional or micron topography, results provided the first evidence of stagnant confluent cell densities on nanostructured surfaces at time points between 1 and 7 days. Moreover, compared to surfaces with a conventional topography, increased chondrocyte intracellular synthesis of alkaline phosphatase and chondrocyte expressed protein-68 (proteins that have been correlated with the functions of chondrocytes) were observed on nanophase PLGA/nanophase titania composites. The present study, thus, provided the first evidence of different chondrocyte responses to nanostructured PLGA/nanophase titania composites; in light of other reports demonstrating increased functions of bone cells on the same materials, such data indicates that further investigation of these materials at the bone-cartilage interface should be conducted.
在本体外研究中，对新型纳米相聚乳酸/乙醇酸共聚物（PLGA）和二氧化钛复合材料上的软骨细胞（合成软骨的细胞）密度以及软骨细胞合成特定细胞内蛋白质的情况进行了研究。通过用10N氢氧化钠对传统（或微米结构）PLGA薄膜进行1小时的化学处理来制备纳米相PLGA薄膜。通过使用传统（即微米级）或纳米级粒径的二氧化钛来控制陶瓷压块中二氧化钛颗粒的尺寸。还制备了传统或纳米相PLGA与传统或纳米相二氧化钛按7Wt%/30wt%比例组成的复合材料。与具有传统或微米形貌的表面相比，结果首次证明在1至7天的时间点，纳米结构表面上的汇合细胞密度停滞。此外，与具有传统形貌的表面相比，在纳米相PLGA/纳米相二氧化钛复合材料上观察到软骨细胞内碱性磷酸酶和软骨细胞表达蛋白-68（与软骨细胞功能相关的蛋白质）的合成增加。因此，本研究首次证明了软骨细胞对纳米结构PLGA/纳米相二氧化钛复合材料有不同反应；鉴于其他报告表明相同材料上骨细胞的功能增强，此类数据表明应在骨-软骨界面进一步研究这些材料。
