Montalbano Giorgia, Borciani Giorgia, Cerqueni Giorgia, Licini Caterina, Banche-Niclot Federica, Janner Davide, Sola Stefania, Fiorilli Sonia, Mattioli-Belmonte Monica, Ciapetti Gabriela, Vitale-Brovarone Chiara
Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
Scienze e Tecnologie Biomediche, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy.
Nanomaterials (Basel). 2020 Aug 27;10(9):1681. doi: 10.3390/nano10091681.
Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to reproduce the physiological environment: By mimicking the natural features of bone tissue, the cell behavior during the regeneration process may be addressed. At present, 3D-printing technologies are the only techniques able to design complex structures avoiding constraints of final shape and porosity. However, this type of biofabrication requires complex optimization of biomaterial formulations in terms of specific rheological and mechanical properties while preserving high biocompatibility. In this work, we combined nano-sized mesoporous bioactive glasses enriched with strontium ions with type I collagen, to formulate a bioactive ink for 3D-printing technologies. Moreover, to avoid the premature release of strontium ions within the crosslinking medium and to significantly increase the material mechanical and thermal stability, we applied an optimized chemical treatment using ethanol-dissolved genipin solutions. The high biocompatibility of the hybrid system was confirmed by using MG-63 and Saos-2 osteoblast-like cell lines, further highlighting the great potential of the innovative nanocomposite for the design of bone-like scaffolds.
由仿生生物活性材料诱导的骨组织再生是治疗由创伤或骨质疏松等疾病引起的骨质流失的临床方法中最具前景的替代方法。目标是设计能够重现生理环境的纳米结构生物活性构建体：通过模仿骨组织的自然特征，可以调控再生过程中的细胞行为。目前，3D打印技术是唯一能够设计复杂结构而不受最终形状和孔隙率限制的技术。然而，这种生物制造类型需要在保持高生物相容性的同时，针对特定的流变学和机械性能对生物材料配方进行复杂的优化。在这项工作中，我们将富含锶离子的纳米级介孔生物活性玻璃与I型胶原蛋白相结合，以配制用于3D打印技术的生物活性墨水。此外，为避免锶离子在交联介质中过早释放，并显著提高材料的机械和热稳定性，我们使用乙醇溶解的京尼平溶液进行了优化的化学处理。通过使用MG-63和Saos-2成骨样细胞系证实了该混合系统的高生物相容性，进一步突出了这种创新纳米复合材料在设计骨样支架方面的巨大潜力。
