转基因小鼠和大鼠中的小干扰RNA与基因沉默

Small interfering RNA and gene silencing in transgenic mice and rats.

作者信息

Hasuwa Hidetoshi, Kaseda Kazuhiro, Einarsdottir Thorbjorg, Okabe Masaru

机构信息

Genome Information Research Center, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan.

出版信息

FEBS Lett. 2002 Dec 4;532(1-2):227-30. doi: 10.1016/s0014-5793(02)03680-3.

DOI:10.1016/s0014-5793(02)03680-3

PMID:12459495

Abstract

After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.

摘要

在合成的21 - 23个核苷酸的短双链RNA（siRNA）被报道可有效沉默特定基因后，一种基于载体的siRNA方法在哺乳动物培养细胞系中得到了证实。然而，RNA干扰（RNAi）对活体动物中各种分化细胞的影响仍不清楚。在本报告中，我们证明转基因提供的siRNA可以在小鼠和大鼠身体的各个部位沉默普遍表达的增强型绿色荧光蛋白。这些结果表明，转基因RNAi可以通过对胚胎干细胞（ES）应用同源重组作为基因沉默的替代方法，并且即使在尚未建立ES细胞系的物种中也应该会成功。