Fujimura S, Kawamura T, Kato S, Tateno H, Watanabe A
Departments of Microbiology and Gastroenterology, Miyagi University, Miyagi prefecture, Japan.
Lett Appl Microbiol. 2002;35(6):504-7. doi: 10.1046/j.1472-765x.2002.01229.x.
To investigate the existence of Helicobacter pylori in cow's milk as one of the foods which most Japanese children eat.
Detection of H. pylori was demonstrated by the semi-nested polymerase chain reaction (PCR), a culture method and electron microscopy. Semi-nested PCR demonstrated the ureA gene of H. pylori in 13 of 18 (72.2%) raw milk samples and in 11 of 20 (55%) commercial pasteurized milk samples. Helicobacter pylori binding immunomagnetic beads with H. pylori-specific goat anti-H. pylori antibody was shown by electron microscopy in both raw and pasteurized milk positive for the ureA gene. Helicobacter pylori was cultured in one raw milk sample, whereas it was not cultured in pasteurized milk samples.
CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: There is a possibility that cow's milk is a transmission vehicle in childhood H. pylori infection, although we failed to confirm the survival of H. pylori in pasteurized milk.
调查牛奶中幽门螺杆菌的存在情况，牛奶是大多数日本儿童食用的食物之一。
通过半巢式聚合酶链反应（PCR）、培养法和电子显微镜检测幽门螺杆菌。半巢式PCR在18份生牛奶样本中的13份（72.2%）以及20份市售巴氏杀菌牛奶样本中的11份（55%）中检测到幽门螺杆菌的ureA基因。通过电子显微镜观察发现，在ureA基因呈阳性的生牛奶和巴氏杀菌牛奶中，幽门螺杆菌特异性山羊抗幽门螺杆菌抗体结合免疫磁珠。在一份生牛奶样本中培养出了幽门螺杆菌，而在巴氏杀菌牛奶样本中未培养出。
研究的结论、意义及影响：尽管我们未能证实在巴氏杀菌牛奶中幽门螺杆菌能够存活，但牛奶有可能是儿童幽门螺杆菌感染的传播媒介。
