为细胞生物学创建新型荧光探针。

Creating new fluorescent probes for cell biology.

作者信息

Zhang Jin, Campbell Robert E, Ting Alice Y, Tsien Roger Y

机构信息

Department of Pharmacology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 18-496, Cambridge, Massachusetts 02139, USA.

出版信息

Nat Rev Mol Cell Biol. 2002 Dec;3(12):906-18. doi: 10.1038/nrm976.

DOI:10.1038/nrm976

PMID:12461557

Abstract

Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.

摘要

荧光探针是活细胞实时成像的基石之一，也是细胞生物学家的有力工具。它们具有高灵敏度和广泛的通用性，同时对被研究细胞的干扰最小。源自荧光蛋白的基因编码报告构建体正在引领各种细胞事件实时可视化和追踪的一场革命。最近的进展包括持续开发用于测量活细胞中生物分子表达和定位的“被动”标记，以及用于监测更复杂细胞过程（如小分子信使动态、酶激活和蛋白质 - 蛋白质相互作用）的“主动”指示剂。