迈向生物材料的实用软X射线光谱显微镜技术。

Towards practical soft X-ray spectromicroscopy of biomaterials.

作者信息

Hitchcock A P, Morin C, Heng Y M, Cornelius R M, Brash J L

机构信息

Brockhouse Institute for Materials Research, McMaster University, Hamilton, ON, Canada, L8S 4MI.

出版信息

J Biomater Sci Polym Ed. 2002;13(8):919-37. doi: 10.1163/156856202320401960.

DOI:10.1163/156856202320401960

PMID:12463511

Abstract

Scanning transmission X-ray microscopy (STXM) is being developed as a new tool to study the surface chemical morphology and biointeractions of candidate biomaterials with emphasis on blood compatible polymers. STXM is a synchrotron based technique which provides quantitative chemical mapping at a spatial resolution of 50 nm. Chemical speciation is provided by the near edge X-ray absorption spectral (NEXAFS) signal. We show that STXM can detect proteins on soft X-ray transparent polymer thin films with monolayer sensitivity. Of great significance is the fact that measurements can be made in situ, i.e. in the presence of an overlayer of the protein solution. The strengths, limitations and future potential of STXM for studies of biomaterials are discussed.

摘要

扫描透射X射线显微镜（STXM）正作为一种新工具进行开发，用于研究候选生物材料的表面化学形态和生物相互作用，重点是血液相容性聚合物。STXM是一种基于同步加速器的技术，可在50纳米的空间分辨率下提供定量化学图谱。化学形态由近边X射线吸收光谱（NEXAFS）信号提供。我们表明，STXM能够以单层灵敏度检测软X射线透明聚合物薄膜上的蛋白质。非常重要的一点是，可以原位进行测量，即在蛋白质溶液覆盖层存在的情况下进行测量。本文讨论了STXM在生物材料研究中的优势、局限性和未来潜力。