Pramila Tata, Miles Shawna, GuhaThakurta Debraj, Jemiolo Dave, Breeden Linda L
Fred Hutchinson Cancer Research Center, Basic Sciences Division, Seattle, Washington 98109-1024, USA.
Genes Dev. 2002 Dec 1;16(23):3034-45. doi: 10.1101/gad.1034302.
Two homeodomain proteins, Yox1 and Yhp1, act as repressors at early cell cycle boxes (ECBs) to restrict their activity to the M/G1 phase of the cell cycle in budding yeast. These proteins bind to Mcm1 and to a typical homeodomain binding site. The expression of Yox1 is periodic and directly correlated with its binding to, and repression of, ECB activity. The absence of Yox1 and Yhp1 or the constitutive expression of Yox1 leads to the loss of cell-cycle regulation of ECB activity. Therefore, the cell-cycle-regulated expression of these repressors defines the interval of ECB-dependent transcription. Twenty-eight genes, including MCM2-7, CDC6, SWI4, CLN3, and a number of genes required during late M phase have been identified that are coordinately regulated by this pathway.
两种同源结构域蛋白Yox1和Yhp1在早期细胞周期框（ECB）处作为阻遏物发挥作用，将其活性限制在芽殖酵母细胞周期的M/G1期。这些蛋白与Mcm1以及典型的同源结构域结合位点结合。Yox1的表达具有周期性，且与其对ECB活性的结合及抑制直接相关。Yox1和Yhp1的缺失或Yox1的组成型表达会导致ECB活性的细胞周期调控丧失。因此，这些阻遏物的细胞周期调控表达定义了ECB依赖性转录的时间间隔。已鉴定出28个基因，包括MCM2 - 7、CDC6、SWI4、CLN3以及M期后期所需的一些基因，它们受该途径的协调调控。
