漂白光对通过荧光光漂白恢复法测量细胞膜中横向扩散的影响。
Effect of bleaching light on measurements of lateral diffusion in cell membranes by the fluorescence photobleaching recovery method.
作者信息
Wolf D E, Edidin M, Dragsten P R
出版信息
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2043-5. doi: 10.1073/pnas.77.4.2043.
Abstract
The Fc receptors for IgE on rat basophil leukemia 2H3 cells were labeled with either rhodamine- or fluorescein-conjugated IgE and then with Fab fragments of anti-IgE conjugated with the other dye. Fluorescence photobleaching recovery measurements of lateral diffusion were performed with one of the dyes before and after extensive bleaching of the other dye over an entire cell. Bleaching one dye did not affect subsequent measurements made with the other dye. We thus detect no evidence for photoinduced artifacts in fluorescence photobleaching recovery experiments.
摘要
用罗丹明或荧光素偶联的IgE标记大鼠嗜碱性粒细胞白血病2H3细胞上的IgE Fc受体，然后用与另一种染料偶联的抗IgE Fab片段进行标记。在对整个细胞上的另一种染料进行广泛漂白之前和之后，用其中一种染料进行侧向扩散的荧光漂白恢复测量。漂白一种染料不影响随后用另一种染料进行的测量。因此，我们在荧光漂白恢复实验中未检测到光诱导伪像的证据。
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