Robles-Sikisaka Refugio, Hasson Kenneth W, Garcia Denise K, Brovont Katherine E, Cleveland Karyn D, Klimpel Kurt R, Dhar Arun K
Department of Biological Sciences, California State University, 333 South Twin Oaks Valley Road, San Marcos, California 92096, USA1.
Super Shrimp Inc., 1545 Tidelands Avenue, Suite J, National City, California 91950, USA2.
J Gen Virol. 2002 Dec;83(Pt 12):3123-3130. doi: 10.1099/0022-1317-83-12-3123.
Taura syndrome virus (TSV) is an important virus infecting penaeid shrimp in the western hemisphere. Genetic variation and immunohistochemical differences of 20 TSV isolates collected from the USA, Taiwan, Mexico and Nicaragua were compared. Capsid protein genes CP1 (546 bp) and CP2 (584 bp) were amplified by RT-PCR and the cDNAs were sequenced. Pairwise comparison of nucleotide sequences showed a 0-2.4% difference in CP1 and a 0-3.5% difference in CP2. Phylogenetic analyses clustered the TSV isolates into two groups: one contained USA, Taiwan and some Mexican isolates, the other contained Mexican isolates only. Immunohistochemical analysis using a TSV-specific monoclonal antibody produced positive results for the USA and Taiwan isolates but negative results for the Mexican and Nicaraguan isolates. Molecular and immunohistochemical data suggest the existence of at least two TSV strains, one of which might have evolved following contact with a new penaeid host, Penaeus stylirostris.
桃拉综合征病毒（TSV）是西半球一种感染对虾的重要病毒。对从美国、台湾、墨西哥和尼加拉瓜收集的20株TSV分离株的遗传变异和免疫组化差异进行了比较。通过RT-PCR扩增衣壳蛋白基因CP1（546 bp）和CP2（584 bp），并对cDNA进行测序。核苷酸序列的两两比较显示，CP1的差异为0-2.4%，CP2的差异为0-3.5%。系统发育分析将TSV分离株分为两组：一组包含美国、台湾和一些墨西哥分离株，另一组仅包含墨西哥分离株。使用TSV特异性单克隆抗体进行的免疫组化分析对美国和台湾分离株产生阳性结果，而对墨西哥和尼加拉瓜分离株产生阴性结果。分子和免疫组化数据表明至少存在两种TSV毒株，其中一种可能是在与新的对虾宿主——斯氏对虾接触后进化而来的。
