Carvalho Pinto-Silva C R, Ferreira J F, Costa R H R, Belli Filho P, Creppy E E, Matias W G
Department of Environmental Engineering, Toxicological Laboratory of the Federal University of Santa Catarina, Brazil.
Toxicon. 2003 Jan;41(1):93-7. doi: 10.1016/s0041-0101(02)00214-3.
Some toxins present in the marine environment are capable of inducing mutagenicity and/or carcinogenicity. Among these toxins, okadaic acid (OA) is gaining considerable interest since it induces DNA based modifications at low concentrations and accumulates in filter-feeding marine animals, including those used for human consumption. This study aims to evaluate the genotoxicity of OA in the haemocytes of the mussel Perna perna, using the micronucleus assay. Fifty-four mussels were separated into three groups of 18 animals. One group received 0.3 microg of OA diluted in 10 microl of ethanol and ultrapure water while the other groups were considered as controls and were exposed to a solvent plus seawater mixture. A significantly higher frequency of micronuclei was observed in haemocytes from the OA-exposed group. There were no statistical differences between the two control groups.
海洋环境中存在的一些毒素能够诱发致突变性和/或致癌性。在这些毒素中，冈田酸（OA）引起了相当大的关注，因为它在低浓度下就能诱导基于DNA的修饰，并在滤食性海洋动物体内积累，包括供人类食用的那些动物。本研究旨在使用微核试验评估OA对贻贝Perna perna血细胞的遗传毒性。54只贻贝被分成三组，每组18只。一组接受在10微升乙醇和超纯水中稀释的0.3微克OA，而其他组作为对照组，暴露于溶剂加海水的混合物中。在暴露于OA的组的血细胞中观察到微核频率显著更高。两个对照组之间没有统计学差异。
