Awal Ram Prasad, Garcia Ronald, Gemperlein Katja, Wink Joachim, Kunwar Bikram, Parajuli Niranjan, Müller Rolf
Department of Pharmaceutical Biotechnology, Saarland University, Building E8 1, D-66123 Saarbrücken, Germany.
Department of Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University Campus Building E 8 1, 66123 Saarbrücken, Germany.
Int J Syst Evol Microbiol. 2017 May;67(5):1422-1430. doi: 10.1099/ijsem.0.001829. Epub 2017 Jun 5.
Bacterial strains MCy10943T and MCy10944T were isolated in 2014 from dried Nepalese soil samples collected in 2013 from Phukot, Kalikot, Western Nepal, and Godawari, Lalitpur, Central Nepal. The novel organisms showed typical myxobacterial growth characteristics, which include swarming colony and fruiting body formation on solid surfaces, and a predatory ability to lyse micro-organisms. The strains were aerobic, mesophilic, chemoheterotrophic and showed resistance to various antibiotics. The major cellular fatty acids common to both organisms were C17 : 0 2-OH, iso-C15 : 0, C16 : 1 and iso-C17 : 0. The G+C content of the genomic DNA was 72-75 mol%. Phylogenetic analysis showed that the strains belong to the family Cystobacteraceae, suborder Cystobacterineae, order Myxococcales. The 16S rRNA gene sequences of both strains showed 97-98 % similarity to Archangium gephyra DSM 2261T andCystobacter violaceus DSM 14727T, and 96.7-97 % to Cystobacter fuscus DSM 2262T and Angiococcus disciformis DSM 52716T. Polyphasic taxonomic characterization suggested that strains MCy10943T and MCy10944T represent two distinct species of a new genus, for which the names Vitiosangium cumulatum gen. nov., sp. nov. and Vitiosangium subalbum sp. nov. are proposed. The type strain of Vitiosangium cumulatum is MCy10943T (=DSM 102952T=NCCB 100600T) while that for Vitiosangium subalbum is MCy10944T (=DSM 102953T=NCCB 100601T). In addition, emended descriptions of the genera Archangium and Angiococcus, and of the family Cystobacteraceaeare provided.
细菌菌株MCy10943T和MCy10944T于2014年从2013年采集的尼泊尔干燥土壤样本中分离得到,这些样本分别采自尼泊尔西部卡利科特的普科特以及尼泊尔中部拉利特布尔的戈达瓦里。这些新发现的微生物呈现出典型的黏细菌生长特征,包括在固体表面形成群体菌落和子实体,以及具有裂解微生物的捕食能力。这些菌株为需氧菌、嗜温菌、化能异养菌,并且对多种抗生素具有抗性。两种微生物共有的主要细胞脂肪酸为C17 : 0 2-OH、异-C15 : 0、C16 : 1和异-C17 : 0。基因组DNA的G+C含量为72 - 75摩尔%。系统发育分析表明,这些菌株属于孢囊杆菌科、孢囊杆菌亚目、黏球菌目。两种菌株的16S rRNA基因序列与盖氏原囊菌DSM 2261T和紫色孢囊杆菌DSM 14727T的相似性为97 - 98%,与暗褐孢囊杆菌DSM 2262T和盘状血管球菌DSM 52716T的相似性为96.7 - 97%。多相分类学特征表明,菌株MCy10943T和MCy10944T代表一个新属的两个不同物种,为此提出新属名为聚积有害黏菌属(Vitiosangium cumulatum)、新种名为聚积有害黏菌(Vitiosangium cumulatum gen. nov., sp. nov.)以及新种名为近白色有害黏菌(Vitiosangium subalbum sp. nov.)。聚积有害黏菌的模式菌株为MCy10943T(=DSM 102952T=NCCB 100600T),而近白色有害黏菌的模式菌株为MCy10944T(=DSM 102953T=NCCB 100601T)。此外,还提供了对原囊菌属和血管球菌属以及孢囊杆菌科的修订描述。