Awal Ram Prasad, Garcia Ronald, Müller Rolf
Department of Pharmaceutical Biotechnology, Saarland University, Building E8 1, 66123 Saarbrücken, Germany.
Department of Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University Campus, Building E 8 1, 66123 Saarbrücken, Germany.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2389-2395. doi: 10.1099/ijsem.0.001045. Epub 2016 Apr 5.
A novel bacterial strain designated MSr9521T was isolated in 2014 from a soil sample collected in 1986 from the Philippines. The novel bacterium shows myxobacterial characteristics that include pseudoplasmodial swarming, fruiting body formation and predatory ability to lyse microorganisms. The strain is chemoheterotrophic, mesophilic and aerobic. Major fatty acids are C18:1, C17:1 2-OH and iso-C15:0, and also contains trace amounts of omega-3/-6 polyunsaturated fatty acids. The G+C content of the genomic DNA is 70.4 mol%. The 16S rRNA gene sequence shows 95-96 % closest similarity to Sorangium cellulosum DSM 14627T, Polyangium fumosum Pl fu5T, Jahnella thaxteri Pl t4T and Byssovorax cruenta By c2T. The molecular phylogenetic analysis shows that the novel isolate forms a novel branch in the family Polyangiaceae, suborder Sorangiineae. Polyphasic taxonomic characterization suggests that the strain MSr9521T represents a novel species of a new genus in the family Polyangiaceae, for which the name Racemicystis crocea gen. nov., sp. nov. is proposed. The type strain of Racemicystis crocea is MSr9521T (=DSM 100773T=NCCB 100574T).
2014年，从1986年采集自菲律宾的一份土壤样本中分离出了一株名为MSr9521T的新型细菌菌株。这种新型细菌表现出粘细菌的特征，包括假原质团游动、子实体形成以及裂解微生物的捕食能力。该菌株为化能异养型、嗜温需氧菌。主要脂肪酸为C18:1、C17:1 2-OH和异-C15:0，还含有微量的ω-3/ω-6多不饱和脂肪酸。基因组DNA的G+C含量为70.4 mol%。16S rRNA基因序列与纤维堆囊菌DSM 14627T、烟色多囊菌Pl fu5T、萨克氏雅内氏菌Pl t4T和食棉铃虫拜氏杆菌By c2T的相似度最高，为95-96%。分子系统发育分析表明，该新分离菌株在多囊菌科、堆囊菌亚目中形成了一个新的分支。多相分类特征表明，菌株MSr9521T代表了多囊菌科中一个新属的一个新物种，为此提出了新属名“Racemicystis”（消旋囊菌属）及新种名“Racemicystis crocea”（黄色消旋囊菌）。黄色消旋囊菌的模式菌株为MSr9521T（=DSM 100773T=NCCB 100574T）。
