温度对精子运动特性和活性氧的影响。

Effects of temperature on sperm motion characteristics and reactive oxygen species.

作者信息

Esfandiari Navid, Saleh Ramadan A, Blaut Andrew P, Sharma Rakesh K, Nelson David R, Thomas Anthony J, Falcone Tommaso, Agarwal Ashok

机构信息

Center for Advanced Research in Human Reproduction Infertility, and Sexual Function, Urological Institute, and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.

出版信息

Int J Fertil Womens Med. 2002 Sep-Oct;47(5):227-33.

PMID:12469709

Abstract

OBJECTIVE

To evaluate the effect of temperature on sperm motility, sperm motion characteristics, and production of reactive oxygen species (ROS) in semen.

METHODS

We collected semen samples from 12 infertile patients and 12 healthy donors and divided each sample into four aliquots just after liquefaction. One aliquot was analyzed immediately after liquefaction and the remaining three were analyzed after one hour of incubation at 4 degrees C, 25 degrees C, and 37 degrees C. Sperm motility and motion characteristics were measured by computer-assisted sperm analysis (CASA). Levels of ROS were determined in washed sperm suspensions using a chemiluminescence assay and results were expressed as x10(6) counted photons per minute (cpm)/20 million sperm/mL.

RESULTS

Motility, curvilinear velocity, straight-line velocity, average path velocity, and amplitude of lateral head displacement decreased significantly from baseline values in both the patients and donors after incubation at 4 degrees C. Percentage motility was highest and ROS levels were lowest in the samples that were incubated at 37 degrees C.

CONCLUSION

Based on our results, we suggest that semen samples be stored at 37 degrees C after collection and during transportation and processing.

摘要

目的

评估温度对精液中精子活力、精子运动特征及活性氧（ROS）产生的影响。

方法

我们收集了12例不育患者和12名健康供者的精液样本，液化后立即将每个样本分成四份。一份在液化后立即进行分析，其余三份在4℃、25℃和37℃孵育1小时后进行分析。精子活力和运动特征通过计算机辅助精子分析（CASA）进行测量。使用化学发光分析法测定洗涤后的精子悬液中的ROS水平，结果以每分钟计数的光子数×10(6)（cpm）/每毫升2000万个精子表示。