Lee Christina J, Huie Philip, Leng Theodore, Peterman Mark C, Marmor Michael F, Blumenkranz Mark S, Bent Stacey F, Fishman Harvey A
Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Arch Ophthalmol. 2002 Dec;120(12):1714-8. doi: 10.1001/archopht.120.12.1714.
To demonstrate that microcontact printing, a modern materials fabrication technique, can be used to engineer the surface of human tissue and to show that inhibitory molecules can be used to pattern the growth of retinal pigment epithelial cells or iris pigment epithelial cells on human lens capsule for transplantation.
Photolithographic techniques were used to fabricate photoresist-coated silicon substrates into molds. Poly(dimethylsiloxane)stamps for microcontact printing were made from these molds. The poly(dimethylsiloxane) stamps were then used to "wet-transfer" growth inhibitory molecules to the surface of prepared human lens capsules that were obtained during cataract surgery. Human retinal pigment epithelial and rabbit iris pigment epithelial cells were grown on a lens capsule substrate in the presence and absence of a patterned array of inhibitory factors.
We found that human lens capsule could be microprinted with a precision similar to that obtained on glass or synthetic polymers. Retinal pigment epithelial cells and iris pigment epithelial cells cultured onto an untreated lens capsule showed spreading and formed into fusiform-appearing cells. In contrast, cells cultured on a lens capsule with a hexagonal micropattern of growth inhibitory molecules retained an epithelioid form within the inhibitory hexagons.
Inhibitory growth molecules can be micropatterned onto human lens capsule, and these micropatterns can control the organization of retinal pigment epithelial cells or iris pigment epithelial cells cultured onto the lens capsule surface.
Microprinting on autologous human tissue may facilitate efforts to effectively organize cell cultures and transplantations for the replacement of vital ocular tissues such as the retinal pigment epithelium in age-related macular degeneration.
证明微接触印刷这种现代材料制造技术可用于构建人体组织表面，并表明抑制性分子可用于在人晶状体囊膜上对视网膜色素上皮细胞或虹膜色素上皮细胞的生长进行图案化处理，以用于移植。
采用光刻技术将涂有光刻胶的硅基板制作为模具。用这些模具制作用于微接触印刷的聚二甲基硅氧烷印章。然后使用聚二甲基硅氧烷印章将生长抑制分子“湿转移”到在白内障手术中获取的已制备人晶状体囊膜表面。在存在和不存在图案化抑制因子阵列的情况下，将人视网膜色素上皮细胞和兔虹膜色素上皮细胞培养在晶状体囊膜基质上。
我们发现人晶状体囊膜可以进行微印刷，其精度与在玻璃或合成聚合物上获得的精度相似。培养在未处理的晶状体囊膜上的视网膜色素上皮细胞和虹膜色素上皮细胞呈铺展状，并形成梭形细胞。相比之下，培养在具有六边形生长抑制分子微图案的晶状体囊膜上的细胞在抑制性六边形内保持上皮样形态。
抑制性生长分子可以微图案化到人的晶状体囊膜上，并且这些微图案可以控制培养在晶状体囊膜表面的视网膜色素上皮细胞或虹膜色素上皮细胞的组织排列。
在自体人体组织上进行微印刷可能有助于有效组织细胞培养和移植，以替代重要的眼部组织，如年龄相关性黄斑变性中的视网膜色素上皮。
